Gene Expression Data
Assay Details
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Assay
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Reference:
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J:68215
Xue Y, et al., Mesodermal patterning defect in mice lacking the Ste20 NCK interacting kinase (NIK). Development. 2001 May;128(9):1559-72
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1933093
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4 C:
embryonic day 8.5
(more  )
Specimen
4 C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4 D:
(close )
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Genetic Background:
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either: (involves: 129S/SvEv * Swiss Webster) or (involves: CD-1 * Swiss Webster)
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Mutant Allele(s):
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Map4k4tm1Eys/Map4k4tm1Eys
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: notochord |
Present |
Regionally restricted |
4D
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Expression only in the notochord and head process.
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| TS13: hindgut diverticulum endoderm |
Absent |
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4D
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Lack of staining in the posterior part of the embryos which corresponds to hindgut endoderm.
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Analysis Tools