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Gene Expression Data
Assay Details
Assay
Reference: J:19631 McKay IJ, et al., The kreisler mouse: a hindbrain segmentation mutant that lacks two rhombomeres. Development. 1994 Aug;120(8):2199-211
Assay type: RNA in situ
MGI Accession ID: MGI:1932351
Gene symbol: Hoxa3
Gene name: homeobox A3
Probe: Mo-10 probe2
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Assay notes: Kreisler -/- mutants at dpc 9.5 have an absence of morphological segmentation posterior to the boundary between r3 and r4.
Results
Specimen 2 H: embryonic day 9.5 (more )
Note: Approximately 22 somites.
Structure Level Pattern Image Note
TS15: rhombomere 1 Absent 2H
TS15: rhombomere 2 Absent 2H
TS15: rhombomere 3 Absent 2H
TS15: rhombomere 4 Absent 2H
TS15: rhombomere 5 Present 2H Expression in posterior regions up to the boundary just anterior to the otocysts and coincident with the boundary betwen r4 and r5.
TS15: neural tube Present Regionally restricted 2H Expression in posterior regions up to the boundary just anterior to the otocysts and coincident with the boundary betwen r4 and r5.

Specimen 2 I: embryonic day 9.5; Mafbkr/Mafbkr (more )
Note: Approximately 22 somites.
Structure Level Pattern Image Note
TS15: rhombomere 1 Absent 2I
TS15: rhombomere 2 Absent 2I
TS15: rhombomere 4 Absent 2I
TS15: rhombomere 3 Present 2I Expression in an ectopic domain in r3.
TS15: rhombomere 5 Present 2I Expression in posterior regions up to the boundary just anterior to the otocysts but this boundary is less sharp than control embryos and reduced r5 expression.
TS15: neural tube Present Regionally restricted 2I Expression in posterior regions up to the boundary just anterior to the otocysts but this boundary is less sharp than control embryos and reduced r5 expression.

Specimen not shown: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: rhombomere 4 Absent
TS15: rhombomere 5 Present Expression in posterior regions up to the boundary just anterior to the otocysts and coincident with the boundary betwen r4 and r5.
TS15: neural tube Present Regionally restricted Expression in posterior regions up to the boundary just anterior to the otocysts and coincident with the boundary betwen r4 and r5.

Specimen not shown: embryonic day 9.5; Mafbkr/Mafbkr (more )
Structure Level Pattern Image Note
TS15: rhombomere 4 Absent
TS15: rhombomere 5 Present Expression in posterior regions up to the boundary just anterior to the otocysts but this boundary is less sharp than control embryos and reduced r5 expression.
TS15: neural tube Present Regionally restricted Expression in posterior regions up to the boundary just anterior to the otocysts but this boundary is less sharp than control embryos and reduced r5 expression.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory