Gene Expression Data
Assay Details
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Assay
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Reference:
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J:44659
Wilson-Rawls J, et al., Differential regulation of epaxial and hypaxial muscle development by paraxis. Development. 1999 Dec;126(23):5217-29
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1930196
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Gene symbol:
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Pax3
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Gene name:
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paired box 3
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Probe:
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Pax3 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
5C:
embryonic day 9.5
(more  )
Specimen
5C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Tcf15tm1Eno/Tcf15tm1Eno
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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Specimen
5E:
embryonic day 10.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk dermomyotome |
Present |
Regionally restricted |
5E
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As somites mature, Pax3 expression becomes more restricted to the dermomyotome, with highest levels in the dorsomedial and dorsolateral lips.
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| TS17: neural tube |
Present |
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5E
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| TS17: forelimb bud |
Present |
Regionally restricted |
5E
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Pax3 transcripts were present in the proximal region of the forelimb buds.
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Tcf15tm1Eno/Tcf15tm1Eno
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk somite |
Present |
Regionally restricted |
5F
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Pax3 expression was present in somites between the forelimbs and hindlimbs.
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| TS17: trunk dermomyotome |
Present |
Regionally restricted |
5F
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A narrow band of Pax3 transcription was present in the dorsolateral half of the dermomyotome.
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| TS17: neural tube |
Present |
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5F
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| TS17: forelimb bud |
Present |
Regionally restricted |
5F
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Pax3 transcripts were present in the proximal region of forelimb buds, although the number of positive cells was significantly reduced.
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Analysis Tools