Gene Expression Data
Assay Details
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Assay
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Reference:
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J:44659
Wilson-Rawls J, et al., Differential regulation of epaxial and hypaxial muscle development by paraxis. Development. 1999 Dec;126(23):5217-29
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1930192
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Gene symbol:
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Myod1
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Gene name:
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myogenic differentiation 1
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Probe:
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Myod cDNA4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3A:
embryonic day 10.5; Myf5tm1Jae/Myf5 +
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Myf5tm1Jae/Myf5+
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: branchial arch |
Present |
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3A
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| TS17: trunk dermomyotome |
Present |
Regionally restricted |
3A
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MyoD was strongly expressed at the dorsomedial edge of the myotome in the cervical somites and the ventrolateral edge of the interlimb somites.
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Tcf15tm1Eno/Tcf15tm1Eno
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: branchial arch |
Present |
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3B
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| TS17: trunk dermomyotome |
Present |
Regionally restricted |
3B
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MyoD transcripts were present in the myotome; the ventrolateral boundary did not extend as far ventrally as in wild type.
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Specimen
3C:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Myf5tm1Jae/Myf5tm1Jae
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S4/SvJae * 129S7/SvEvBrd
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Mutant Allele(s):
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Myf5tm1Jae/Myf5+
Tcf15tm1Eno/Tcf15tm1Eno
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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Specimen
3E:
(close )
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Genetic Background:
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involves: 129S4/SvJae * 129S7/SvEvBrd
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Mutant Allele(s):
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Myf5tm1Jae/Myf5tm1Jae
Tcf15tm1Eno/Tcf15tm1Eno
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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