Probe:	Pdgfa cDNA5
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present		1C
TS11: extraembryonic visceral endoderm	Present		1C
TS11: embryo mesoderm	Weak		1C	Little signal could be seen above the mesoderm.
TS11: ectoplacental cavity	Present		1C	Expressing cells were situated more centrally and closer to the ectoplacental cavity.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Absent		1F
TS11: embryo mesoderm	Weak		1F	Little signal could be seen above the mesoderm.

Structure	Level	Pattern	Image	Note
TS11: chorion ectoderm	Present		1I

Structure	Level	Pattern	Image	Note
TS12: surface ectoderm	Strong		2C/D
TS12: embryo endoderm	Absent		2C/D
TS12: head somite	Weak		2C/D	Somites 2-4.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		2G
TS17: surface ectoderm	Present		2G
TS17: trunk dermomyotome	Present	Regionally restricted	2G	Expression almost exclusively in the myotome part of the dermomyotome.

Structure	Level	Pattern	Image	Note
TS19: neural tube floor plate	Present		2J
TS19: surface ectoderm	Present		2J
TS19: trunk dermomyotome	Present	Regionally restricted	2J	Expression almost exclusively in the myotome part of the dermomyotome.

Structure	Level	Pattern	Image	Note
TS19: future arm ectoderm	Present		3C
TS19: forelimb bud	Present		3C	Expression in the myotome continues into skeletal muscle primordia and into the surface ectoderm of the developing (fore)limb.

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present		3F
TS19: handplate ectoderm	Present		3F

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present	Regionally restricted	4C	Expression in the muscle groups in the forelimb.
TS22: shoulder	Present	Regionally restricted	4C	Expression in the muscle groups in the shoulder.

Structure	Level	Pattern	Image	Note
TS22: axial muscle	Present	Regionally restricted	4G	Expression in three distinct areas corresponding to the intercostal muscles. The authors note that since the first separation of the myotome and dermatome, prospective myoblasts consistently express ligand (Pdgfa) throughout muscle development.

Structure	Level	Pattern	Image	Note
TS22: integumental system	Present		5C
TS22: mammary bud	Present		5C	Expression in the epidermal bud of the developing mammary gland.

Structure	Level	Pattern	Image	Note
TS22: integumental system	Present		5F	Expression in surface ectoderm and in the developing epithelial hair bud.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present		5K	Expression in the surface ectoderm of the mandibular arch.
TS17: pharynx	Present	Regionally restricted	5K	Expression in the endothelium of the pharynx.
TS17: otocyst	Present	Regionally restricted	5K	Expression in the lining of the otic vesicle.
TS17: surface ectoderm	Present		5K	Expression is along the entire surface ectoderm but stops where fusion of the mandibular arches take place.

Structure	Level	Pattern	Image	Note
TS22: submandibular gland primordium epithelium	Present	Regionally restricted	6C	Expression in the branching epithelium buds of the submandibular salivary gland.

Structure	Level	Pattern	Image	Note
TS22: bronchus epithelium	Present		6F	The authors state expression in bronchial epithelium and no specific lobe is indicated.

Structure	Level	Pattern	Image	Note
TS22: bronchus mesenchyme	Present		6H	The authors state expression in bronchial epithelium and no specific lobe is indicated.

Structure	Level	Pattern	Image	Note
TS19: Rathke's pouch	Present		7C	Expression in the presumptive adenohypophesis.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present		7F

Structure	Level	Pattern	Image	Note
TS17: optic stalk	Present	Regionally restricted	8B	Expression in the optic stalk neuroectoderm.
TS17: optic cup	Present	Regionally restricted	8B	Expression in the posterior part of the optic cup.
TS17: surface ectoderm	Present		8B	Expression in the surface ectoderm of the head.

Structure	Level	Pattern	Image	Note
TS17: optic stalk	Present	Regionally restricted	8E	Expression in the optic stalk neuroectoderm.
TS17: optic cup	Present	Regionally restricted	8E	Expression in the posterior part of the optic cup. Expression in the posterior part of the optic cup.
TS17: surface ectoderm	Present		8E	Expression in the surface ectoderm of the head.

Structure	Level	Pattern	Image	Note
TS19: surface ectoderm	Present		8H	Surface ectoderm from which the lens develops.
TS19: optic stalk	Present	Regionally restricted	8H	Expression in the optic stalk neuroectoderm.
TS19: pigmented retinal epithelium	Present		8H
TS19: retina	Present	Regionally restricted	8H	Expression in the posterior part of the optic cup.

Structure	Level	Pattern	Image	Note
TS22: lower eyelid epithelium	Present		8K	Expression in the surface ectoderm of the eyelid.
TS22: upper eyelid epithelium	Present		8K	Expression in the surface ectoderm of the eyelid.