Probe:	Sox3 cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	The authors do not specify which probe was used, either MGI:1928758 or MGI:1928759.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		6A	Expression throughout the neuroectoderm.

Structure	Level	Pattern	Image	Note
TS14: optic vesicle neural ectoderm	Present		6B

Structure	Level	Pattern	Image	Note
TS15: olfactory placode	Present	Regionally restricted	6C
TS15: optic vesicle	Present	Graded	6C	The optic vesicles show a gradient of expression with the lowest level distally.
TS15: future forebrain	Present	Regionally restricted	6C	Expression throughout the neuroectoderm.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	6E , 6D	Expression throughout the neuroectoderm.
TS15: optic vesicle	Present	Graded	6E , 6D	The optic vesicles show a gradient of expression with the lowest levels distally.

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present		6F
TS17: brain	Present		6F
TS17: future spinal cord	Present		6F
TS17: hindbrain	Present	Regionally restricted	6F	Areas of weaker and stronger expression apparently contained within the boundaries of specific rhombomeres.

Structure	Level	Pattern	Image	Note
TS19: brain	Present		6G
TS19: lens vesicle	Absent		6G	Expression is maintained throughout the fetal brain, but not in the optic cup.

Structure	Level	Pattern	Image	Note
TS21: diencephalon lateral wall ventricular layer	Present			Expression is restricted to the ependymal layer.
TS21: midbrain ventricular layer	Present			Expression is restricted to the ependymal layer.
TS21: spinal cord ventricular layer	Present			Expression is restricted to the ependymal layer.