Gene Expression Data
Assay Details
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Assay
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Reference:
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J:41030
Mastick GS, et al., Pax-6 functions in boundary formation and axon guidance in the embryonic mouse forebrain. Development. 1997 May;124(10):1985-97
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1927510
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Gene symbol:
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Dbx1
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Gene name:
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developing brain homeobox 1
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Probe:
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SAH2aSV
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5. I.:
embryonic day 10.5
(more  )
Specimen
5. I.:
(close  )
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Genetic Background:
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FVB
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
5I
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Expression detected in dorsal mesencephalon with a sharp border at the mesencephalon/prosomere (p)1 boundary.
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| TS17: diencephalon |
Present |
Regionally restricted |
5I
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Expression was detected in prosomere 1 and prosomere 2. Heavy expression was detected in ventral prosomere 3.
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| TS17: telencephalon |
Present |
Regionally restricted |
5I
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Expression was detected in prosomere 1 and prosomere 2. Heavy expression was detected in ventral prosomere 3.
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| TS17: rhombomere 1 |
Present |
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5I
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Expression was detected from rhomobomere 1 caudal.
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Specimen
5. I. inset:
embryonic day 10.5
(more )
Specimen
5. I. inset:
(close )
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Genetic Background:
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FVB
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
5I inset
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Expression was detected in dorsal mesencephalon with a sharp border at the mesencephalon/prosomere (p)1 boundary.
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| TS17: diencephalon |
Present |
Regionally restricted |
5I inset
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Expression was detected in prosomere 1 and prosomere 2 in a thin band of ventricular cells that curves from ventral p1 up into dorsal p2.
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Specimen
5. J.:
(close )
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Genetic Background:
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involves: FVB
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
5J
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Expression detected in dorsal mesencephalon, but in mutant expression extends rostrally.
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Specimen
5. J. inset:
(close )
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Genetic Background:
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involves: FVB
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
5J inset
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Expression was primarily in a superficial layer of cells. The mutant expression domain expanded rostrally into what had been prosomere 1, but the gain of expression indicates a shift in the identity of the p1 region to that of mesencephalon.
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