Gene Expression Data
Assay Details
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Assay
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Reference:
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J:63685
Summerbell D, et al., The expression of Myf5 in the developing mouse embryo is controlled by discrete and dispersed enhancers specific for particular populations of skeletal muscle precursors. Development. 2000 Sep;127(17):3745-57
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1888754
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Probe:
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Myf5 genomic6
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1-whole embryo:
embryonic day 9.5
(more  )
Note: Specimens were fixed in Mirsky's fixative.
Specimen
1-whole embryo:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Specimens were fixed in Mirsky's fixative.
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Specimen
1-psm:
embryonic day 9.5
(more )
Note: Specimens were fixed in Mirsky's fixative.
Specimen
1-psm:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Specimens were fixed in Mirsky's fixative.
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Specimen
1-somite I:
embryonic day 9.5
(more )
Note: Specimens were fixed in Mirsky's fixative.
Specimen
1-somite I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Specimens were fixed in Mirsky's fixative.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: myotome |
Strong |
Regionally restricted |
1_I
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Expression detected in the dorsal myotome, and is weak in the ventral part.
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Specimen
1-somite II:
embryonic day 9.5
(more )
Note: Specimens were fixed in Mirsky's fixative.
Specimen
1-somite II:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Specimens were fixed in Mirsky's fixative.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: myotome |
Strong |
Graded |
1_II
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Expression is strong in the dorsal myotome and weak in the ventral myotome.
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Specimen
1-somite IV:
embryonic day 9.5
(more )
Note: Specimens were fixed in Mirsky's fixative.
Specimen
1-somite IV:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Specimens were fixed in Mirsky's fixative.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: myotome |
Strong |
Regionally restricted |
1_IV
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Expression is strong in the ventral myotome and the somitic bud.
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Specimen
1-somite VI:
embryonic day 9.5
(more )
Note: Specimens were fixed in Mirsky's fixative.
Specimen
1-somite VI:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Specimens were fixed in Mirsky's fixative.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: myotome |
Strong |
Regionally restricted |
1_VI
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Expression is strong in the ventral myotome and the somitic bud.
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Specimen
1-somite XIV:
embryonic day 9.5
(more )
Note: Specimens were fixed in Mirsky's fixative.
Specimen
1-somite XIV:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Specimens were fixed in Mirsky's fixative.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: myotome |
Present |
Regionally restricted |
1_XIV
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Expression is detected in the ventral myotome and hypaxial cells that will migrate to the thoracic diaphragm.
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Specimen
1-somite XV:
embryonic day 9.5
(more )
Note: Specimens were fixed in Mirsky's fixative.
Specimen
1-somite XV:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Specimens were fixed in Mirsky's fixative.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: myotome |
Present |
Regionally restricted |
1_XV
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In the section at this level the expression is absent in the ventral myotome cells that will migrate to the limbs.
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Analysis Tools