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Gene Expression Data
Assay Details
Assay
Reference: J:59281 Manaia A, et al., Lmo2 and GATA-3 associated expression in intraembryonic hemogenic sites. Development. 2000 Feb;127(3):643-53
Assay type: RNA in situ
MGI Accession ID: MGI:1352416
Gene symbol: Gata3
Gene name: GATA binding protein 3
Probe: Gata3 cDNA3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1E top panel: embryonic day 8.0 (more )
Note: Late head-fold stage.
Structure Level Pattern Image Note
TS12: gut Present 1E top
TS12: embryo ectoderm Present Regionally restricted 1E top Expression is in the lateral ectoderm.
TS12: allantois Present Regionally restricted 1E top Expression is where hematopoietic cluster-bearing vessels develop.
TS12: splanchnopleure Present Regionally restricted 1E top Expression is where hematopoietic cluster-bearing vessels develop.
Specimen 1E bottom panel: embryonic day 8.0 (more )
Note: Late head-fold stage, 3 somites.
Structure Level Pattern Image Note
TS12: allantois Present 1E bottom
TS12: splanchnopleure Present 1E bottom
TS12: blood island Absent 1E bottom
Specimen 1I: embryonic day 9.5-10.0 (more )
Note: 25-30 somites.
Structure Level Pattern Image Note
TS14: 2nd branchial arch Present 1I Authors state expression is detected in the 2nd, 3rd and 4th Branchial Arches; the aortic region and the Mesonephros.
TS14: 3rd branchial arch Present 1I Authors state expression is detected in the 2nd, 3rd and 4th Branchial Arches; the aortic region and the Mesonephros.
TS14: dorsal aorta Present 1I Authors state expression is detected in the 2nd, 3rd and 4th Branchial Arches; the aortic region and the Mesonephros.
TS14: pronephros Present 1I Authors state expression is detected in the 2nd, 3rd and 4th Branchial Arches; the aortic region and the Mesonephros.
TS14: embryo mesenchyme Present Regionally restricted 1I Expression is in the posterior ventral mesoderm.
Specimen 2E: embryonic day 8.0 (more )
Note: 5-somite stage. Ventral view.
Structure Level Pattern Image Note
TS12: splanchnopleure Present Regionally restricted 2E Expression is caudal.
TS12: allantois Present Regionally restricted 2E Expression is at the basis of the allantois.
Specimen 2F,G: embryonic day 8.0 (more )
Note: 5-somite stage. The section in (F) is truncal and in (G) is caudal.
Structure Level Pattern Image Note
TS12: embryo endoderm Present Regionally restricted 2F , 2G Expression is in the area underlying the aorta.
TS12: embryo ectoderm Present Regionally restricted 2G , 2F Expression is in the lateral ectoderm.
Specimen 3A: embryonic day 10.0-10.5 (more )
Note: This section is from the aortic region.
Structure Level Pattern Image Note
TS16: trunk mesenchyme Present Regionally restricted 3A Expression is in mesodermal cells underlying the aorta. A few cells display a stronger signal.
Specimen 3B: embryonic day 11.0-11.5 (more )
Note: This section is from the aortic region.
Structure Level Pattern Image Note
TS19: dorsal aorta Present Regionally restricted 3B Expression is at the basis of an aortic cluster.
TS19: trunk mesenchyme Present Regionally restricted 3B Expression is within mesodermal patches beneath the aorta.
Specimen 3D: embryonic day 11.0-11.5 (more )
Note: This section is from the aortic region.
Structure Level Pattern Image Note
TS19: dorsal aorta Present Patchy 3D
Specimen 3E: embryonic day 11.0-11.5 (more )
Note: Double labeled: yellow - Gata3; blue - Th (assay MGI:1352435); red - overlap.
Structure Level Pattern Image Note
TS19: dorsal aorta Present Patchy 3E
Specimen 3F: embryonic day 11.0-11.5 (more )
Structure Level Pattern Image Note
TS19: dorsal aorta Present Patchy 3F
Specimen 3G: embryonic day 11.0-11.5 (more )
Structure Level Pattern Image Note
TS19: dorsal aorta Present Patchy 3G
Specimen 3H: embryonic day 11.0-11.5 (more )
Note: Double labeled: yellow - Gata3; blue - Ptprc (assay MGI:1352429); red - overlap.
Structure Level Pattern Image Note
TS19: dorsal aorta Present Patchy 3H
Specimen 4B: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: hepatic primordium Present Uniform 4B This is before hematopoietic colinization.
Specimen 4C: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: liver Strong Spotted 4C
Specimen 4G: embryonic day 10.0 (more )
Note: 30-35 somites.
Structure Level Pattern Image Note
TS16: optic cup Present 4G Expression is in the optic and otic placodes.
TS16: otocyst Present 4G Expression is in the optic and otic placodes.
TS16: 1st branchial arch Present 4G The thymic rudiment is clearly labeled.
TS16: 2nd branchial arch Present 4G The thymic rudiment is clearly labeled.
TS16: 3rd branchial arch Present 4G The thymic rudiment is clearly labeled.
TS16: 4th branchial arch Present 4G The thymic rudiment is clearly labeled.
Specimen 4H top: embryonic day 10.0 (more )
Note: 30-35 somites. Sagittal section
Structure Level Pattern Image Note
TS16: 1st branchial arch mesenchyme Present Regionally restricted 4H top Expression is in the mesoderm.
TS16: 2nd branchial arch mesenchyme Present 4H top Expression is in the mesoderm.
TS16: 3rd branchial arch mesenchyme Present 4H top Expression is in the mesoderm.
TS16: 4th branchial arch mesenchyme Present 4H top Expression is in the mesoderm.
TS16: 3rd branchial pouch endoderm Present Regionally restricted 4H top Expression is restricted to the thymic epithelium.
Specimen 4H bottom: embryonic day 10.0 (more )
Note: 30-35 somites.
Structure Level Pattern Image Note
TS16: 3rd branchial pouch endoderm Present Regionally restricted 4H bottom Expression is on the side of the thymic rudiment opposite the foregut.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory