Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:58081
Lettice L, et al., The dominant hemimelia mutation uncouples epithelial-mesenchymal interactions and disrupts anterior mesenchyme formation in mouse hindlimbs. Development. 1999 Nov;126(21):4729-36
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:1347778
|
Gene symbol:
|
Sox9
|
Gene name:
|
SRY (sex determining region Y)-box 9
|
Probe:
|
Sox9 probe2
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Results
|
Specimen
4G +/+:
embryonic day 11.5
(more )
Specimen
4G +/+:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: footplate mesenchyme |
Present |
Regionally restricted |
4G
|
Expression marks the condensing mesenchyme that will form the skeletal elements.
|
TS19: future leg mesenchyme |
Present |
Regionally restricted |
4G
|
Expression marks the condensing mesenchyme that will form the skeletal elements.
|
Specimen
4H Dh/+:
embryonic day 11.5; Dh/Dh +
(more )
Specimen
4H Dh/+:
(close )
Genetic Background:
|
involves: C57BL/6
|
Mutant Allele(s):
|
Dh/Dh+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: footplate mesenchyme |
Present |
Regionally restricted |
4H
|
Expression is in the condensing mesenchyme that will form the skeletal elements. Expression is similar to wild-type.
|
TS19: future leg mesenchyme |
Present |
Regionally restricted |
4H
|
Expression is in the condensing mesenchyme that will form the skeletal elements. Expression is similar to wild-type.
|
Specimen
4I Dh/Dh:
embryonic day 11.5; Dh/ Dh
(more )
Specimen
4I Dh/Dh:
(close )
Genetic Background:
|
involves: C57BL/6
|
Mutant Allele(s):
|
Dh/Dh
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: footplate mesenchyme |
Present |
Regionally restricted |
4I
|
In mutants there is only half the expression presumably in the fibula. The digital arch is reduced in size.
|
TS19: future leg mesenchyme |
Present |
Regionally restricted |
4I
|
In mutants there is only half the expression presumably in the fibula. The digital arch is reduced in size.
|
|