Gene Expression Data
Assay Details
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Assay
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Reference:
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J:54638
Henderson DJ, et al., Rib truncations and fusions in the Sp2H mouse reveal a role for Pax3 in specification of the ventro-lateral and posterior parts of the somite. Dev Biol. 1999 May 1;209(1):143-58
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1346738
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Gene symbol:
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Tnc
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Gene name:
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tenascin C
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Probe:
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Tenascin-C probe-1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Beta-galactosidase
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Results
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Specimen
5.A wt:
embryonic day 11.5
(more  )
Specimen
5.A wt:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: embryo |
Present |
Regionally restricted |
5A left
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Expression is seen in a segmental pattern in the flank.
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Specimen
5.A Sp2H:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: embryo |
Present |
Regionally restricted |
5A right
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Expression is seen in a segmental pattern in the flank. Expression domain is truncated both dorsally and ventrally relative to the wild-type.
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Specimen
5.C:
embryonic day 11.5
(more )
Note: After whole-mount in situ hybridizations, embryos were sectioned in 0.45% gelatine, 27% albumin, 18% glucose in PBS which was solidified by the addition of 1/10 volume of 30% glutaraldehyde.
Specimen
5.C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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After whole-mount in situ hybridizations, embryos were sectioned in 0.45% gelatine, 27% albumin, 18% glucose in PBS which was solidified by the addition of 1/10 volume of 30% glutaraldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trunk sclerotome |
Present |
Regionally restricted |
5C
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Expression is seen in the lateral sclerotome.
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Specimen
5.D:
embryonic day 11.5; Pax3Sp-2H/ Pax3Sp-2H
(more )
Note: After whole-mount in situ hybridizations, embryos were sectioned in 0.45% gelatine, 27% albumin, 18% glucose in PBS which was solidified by the addition of 1/10 volume of 30% glutaraldehyde.
Specimen
5.D:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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After whole-mount in situ hybridizations, embryos were sectioned in 0.45% gelatine, 27% albumin, 18% glucose in PBS which was solidified by the addition of 1/10 volume of 30% glutaraldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trunk sclerotome |
Present |
Regionally restricted |
5D
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Expression is seen in the lateral sclerotome. The domain of expression is truncated ventrally but expanded mediolaterally relative to the wild-type.
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Specimen
5.E wt:
embryonic day 12.5
(more )
Specimen
5.E wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: embryo |
Present |
Regionally restricted |
5E left
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Expression domain has expanded dorsoventrally and now prefigures the development of the ribs.
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Specimen
5.E Sp2H:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: embryo |
Present |
Regionally restricted |
5E right
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Expression domain has expanded dorsoventrally and now prefigures the development of the ribs. Expression domain is truncated ventrally relative to the wild-type.
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Specimen
not shown wt:
embryonic day 10.5
(more )
Specimen
not shown wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Weak |
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Authors report very low levels of expression.
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Specimen
not shown Sp2H:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Weak |
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Authors report no identifiable differences between wild-type and mutant embryos,
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Analysis Tools