Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:13029
Haack H, et al., The establishment of murine Hox-1 expression domains during patterning of the limb. Dev Biol. 1993 Jun;157(2):410-22
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1344727
|
|
Gene symbol:
|
Hoxa11
|
|
Gene name:
|
homeobox A11
|
|
Probe:
|
pbH1.9-158S-R
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
4C, 1.9:
embryonic day 12.5
(more  )
Specimen
4C, 1.9:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
4C
|
Expression is in a proximodistally-restricted band that does not extend to the distal tip.
|
| TS20: hindlimb |
Present |
Regionally restricted |
4C
|
Expression is in a proximodistally-restricted band that does not extend to the distal tip.
|
Specimen
5D, 1.9:
embryonic day 9.75
(more )
Specimen
5D, 1.9:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.75
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
5D
|
Expression is restricted to the ventral, distal side of the limb. Expression in the posterior half is more extended. Expression is also seem in a region adjacent to the somites. The authors call this somatopleural expression.
|
Specimen
5I, 1.9:
embryonic day 10.5
(more )
Specimen
5I, 1.9:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
5I
|
Expression etends anteriorly, but remains in the ventral half of the limb. Only a small distal region expresses dorsally. A band along the periphery is seen in 5M.
|
Specimen
5M, 1.9:
embryonic day 10.5
(more )
Specimen
5M, 1.9:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
5M
|
Expression etends anteriorly, but remains in the ventral half of the limb. Only a small distal region expresses dorsally. A band along the periphery is seen in 5M.
|
Specimen
5Q, 1.9:
embryonic day 11.0
(more )
Specimen
5Q, 1.9:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
5Q
|
Expression is a mirror image of the expression at TS17. Expression is now in the dorsal side of the limb.
|
Specimen
5U, 1.9:
embryonic day 11.5
(more )
Specimen
5U, 1.9:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
5U
|
Expression extends further anteriorly and has a concave shape proximally. the proximal domain is established and distal expression ceases.
|
Specimen
8A,B:
embryonic day 9.75
(more )
Specimen
8A,B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.75
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: trunk somite |
Present |
Regionally restricted |
8A
|
Expression is in the venrolateral margin of the somites.
|
| TS16: trunk paraxial mesenchyme |
Present |
Regionally restricted |
8B
|
Expression is in the mesenchyme adjacent to the forelimb overlying the coelom.
|
Specimen
8C,D:
embryonic day 10.0
(more )
Specimen
8C,D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: trunk paraxial mesenchyme |
Present |
Regionally restricted |
8C
,
8D
|
Expression in the ventral part of the domain adjacent to the somites (somatopleural) becomes disperse.
|
Specimen
8E,F:
embryonic day 10.5
(more )
Specimen
8E,F:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk paraxial mesenchyme |
Present |
Regionally restricted |
8F
,
8E
|
Expression in the entire domain adjacent to the somite (somatopleural) bcomes disperse.
|
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
8F
,
8E
|
Expression is in the core rather than the peripheral mesenchyme. It appears stronger in two proximodistal stripes near the cephalis and basilic vein.
|
|
Analysis Tools