Gene Expression Data
Assay Details
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Assay
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Reference:
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J:13029
Haack H, et al., The establishment of murine Hox-1 expression domains during patterning of the limb. Dev Biol. 1993 Jun;157(2):410-22
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1344713
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Gene symbol:
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Hoxa10
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Gene name:
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homeobox A10
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Probe:
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pbH1.8-320E-S
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
4B, 1.8:
embryonic day 12.5
(more  )
Specimen
4B, 1.8:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
4B
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Expression is in a proximal domain. Minor asymmetries in expression are found along the anterior-posterior and dorsal-ventral axes. Enhanced expression is seen in a domain that ovelaps with the Hoxa11 domain.
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| TS20: hindlimb |
Present |
Regionally restricted |
4B
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Expression is in a proximal domain. Minor asymmetries in expression are found along the anterior-posterior and dorsal-ventral axes. Enhanced expression is seen in a domain that ovelaps with the Hoxa11 domain.
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Specimen
5B, 1.8:
embryonic day 9.75
(more )
Specimen
5B, 1.8:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Regionally restricted |
5B
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Expression is in a small ventral, distal region. Posterior expression is slightly more extended. Expression is also seen adjacent to the somites. The authors call this the somatopleural domain.
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Specimen
5G, 1.8:
embryonic day 10.5
(more )
Specimen
5G, 1.8:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
5G
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Expression extends anteriorly compared to TS16, but remains in the ventral half of the limb. Expression extends more proximally than expression of Hoxa11.
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Specimen
5K, 1.8:
embryonic day 10.5
(more )
Specimen
5K, 1.8:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
5K
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Expression extends anteriorly compared to TS16, but remains in the ventral half of the limb. Expression extends more proximally than expression of Hoxa11.
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Specimen
5O, 1.8:
embryonic day 11.0
(more )
Specimen
5O, 1.8:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
5O
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Expression extends more proximally than in earlier stages. There is no expression in very proximal and posterior regions.
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Specimen
5S, 1.8:
embryonic day 11.5
(more )
Specimen
5S, 1.8:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
5S
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Expression extends further proximaly than at TS18. There is enhanced expression in the domain that overlaps Hoxa11 expression. There is no expression in the very proximal and posterior region.
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Analysis Tools