Probe:	rat IGF-II cDNA clone 27
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS5: blastocyst	Absent

Structure	Level	Pattern	Image	Note
TS8: ectoplacental cone	Strong		1a
TS8: extraembryonic ectoderm	Strong		1a
TS8: epiblast	Absent		1a
TS8: mural trophectoderm	Ambiguous		1a	The presence of scattered grains outlining the region of the mural trophectoderm were noticed, but the intensity of this signal was not convincingly above background levels.

Structure	Level	Pattern	Image	Note
TS8: ectoplacental cone	Strong		1b
TS8: extraembryonic ectoderm	Strong		1b
TS8: epiblast	Absent		1b
TS8: mural trophectoderm	Ambiguous		1b	The presence of scattered grains outlining the region of the mural trophectoderm were noticed, but the intensity of this signal was not convincingly above background levels.

Structure	Level	Pattern	Image	Note
TS10: ectoplacental cone	Present		1c
TS10: extraembryonic ectoderm	Present		1c
TS10: parietal endoderm	Present		1c
TS10: extraembryonic visceral endoderm	Present		1c	Expression is present in the columnar visceral epithelium. In constrast, the occasional labelling in the region of squamous endoderm was never convincingly above background levels.
TS10: embryo mesoderm	Absent		1c
TS10: amniotic fold mesoderm	Strong		1c

Structure	Level	Pattern	Image	Note
TS10: ectoplacental cone	Present		1d
TS10: extraembryonic ectoderm	Present		1d
TS10: parietal endoderm	Present		1d
TS10: extraembryonic visceral endoderm	Present		1d	Expression is present in the columnar visceral epithelium. In constrast, the occasional labelling in the region of squamous endoderm was never convincingly above background levels.
TS10: embryo mesoderm	Absent		1d
TS10: amniotic fold mesoderm	Strong		1d

Structure	Level	Pattern	Image	Note
TS11: allantois	Present		1e
TS11: chorion ectoderm	Present		1e
TS11: chorion mesoderm	Present		1e
TS11: yolk sac endoderm	Present		1e
TS11: yolk sac mesoderm	Present		1e
TS11: amnion mesoderm	Present		1e
TS11: primitive streak	Absent		1e
TS11: embryo mesoderm	Present	Regionally restricted	1e	A girdle of labelling was present in the proximal mesodermal wings, excluding the primitive streak, in the form of a horse-shoe surrounding the remaining proximal area of the epiblast. Signal was localized in the anterior-proximal embryonic mesoderm.

Structure	Level	Pattern	Image	Note
TS11: allantois	Present		1f
TS11: chorion ectoderm	Present		1f
TS11: chorion mesoderm	Present		1f
TS11: yolk sac endoderm	Present		1f

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Absent		1g
TS11: embryo mesoderm	Present	Regionally restricted	1g	A girdle of labelling was present in the proximal mesodermal wings, excluding the primitive streak, in the form of a horse-shoe surrounding the remaining proximal area of the epiblast. Signal was localized in the anterior-proximal embryonic mesoderm.

Structure	Level	Pattern	Image	Note
TS10: mural trophectoderm	Present		1h
TS10: primary trophoblast giant cell	Present		1h

Structure	Level	Pattern	Image	Note
TS11: amnion mesoderm	Present		1i

Structure	Level	Pattern	Image	Note
TS11: amnion ectoderm	Absent			On the basis of a rare unequivocal result showing exclusive localization of labelling in the mesodermal layer of the amnion, it is believed that the IGF-II gene is not expressed in the amniotic ectoderm.

Structure	Level	Pattern	Image	Note
TS12: amnion	Strong		2a
TS12: allantois	Strong		2a
TS12: heart	Strong		2a
TS12: head mesenchyme	Strong		2a
TS12: embryo mesenchyme	Trace	Regionally restricted	2a	Labeling was detected at background levels in the midline.
TS12: foregut diverticulum endoderm	Strong	Regionally restricted	2a	The hybridization signal was localized in the ventral and lateral walls, whereas the dorsal wall was practically unlabeled at this stage.

Structure	Level	Pattern	Image	Note
TS12: heart	Strong		2c
TS12: foregut diverticulum endoderm	Strong	Regionally restricted	2c	The hybridization signal was localized in the ventral and lateral walls, whereas the dorsal wall was practically unlabeled at this stage.

Structure	Level	Pattern	Image	Note
TS12: amnion	Strong		2d
TS12: somatopleure	Strong		2d
TS12: splanchnopleure	Strong		2d

Structure	Level	Pattern	Image	Note
TS12: ectoplacental cone	Weak		2e
TS12: parietal endoderm	Absent		2e
TS12: yolk sac endoderm	Absent		2e	Signal was completely eliminated in the outer endodermal layer of the visceral yolk sac.
TS12: extraembryonic component	Present	Regionally restricted	2e	Extraembryonic mesoderm continued to exhibit a positive signal.
TS12: blood island	Absent		2e
TS12: heart	Strong		2e
TS12: foregut diverticulum endoderm	Strong	Regionally restricted	2e	The hybridization signal was localized in the ventral and lateral walls, whereas the dorsal wall was practically unlabeled at this stage.

Structure	Level	Pattern	Image	Note
TS12: somatopleure	Present		2f
TS12: splanchnopleure	Present		2f

Structure	Level	Pattern	Image	Note
TS12: head mesenchyme	Present		2g

Structure	Level	Pattern	Image	Note
TS12: allantois	Strong		2i
TS12: amnion	Strong		2i
TS12: ectoplacental cone	Strong	Non-Uniform	2i	The walls of the ectoplacental cavity, which had almost completely collapsed, continued to exhibit a strong signal. The remaining ectoplacental cone has weak staining.
TS12: parietal endoderm	Absent		2i
TS12: yolk sac endoderm	Absent		2i	Signal was completely eliminated in the outer endodermal layer of the visceral yolk sac.
TS12: extraembryonic component	Present	Regionally restricted	2i	Extraembryonic mesoderm continued to exhibit a positive signal.
TS12: blood island	Absent		2i
TS12: heart	Strong		2i
TS12: head mesenchyme	Strong		2i
TS12: foregut diverticulum endoderm	Strong	Regionally restricted	2i	The hybridization signal was localized in the ventral and lateral walls, whereas the dorsal wall was practically unlabeled at this stage.

Structure	Level	Pattern	Image	Note
TS12: heart	Strong		2j
TS12: foregut diverticulum endoderm	Strong	Regionally restricted	2j	The hybridization signal was localized in the ventral and lateral walls, whereas the dorsal wall was practically unlabeled at this stage.

Structure	Level	Pattern	Image	Note
TS12: head paraxial mesenchyme	Trace		3a

Structure	Level	Pattern	Image	Note
TS12: head paraxial mesenchyme	Trace		3b

Structure	Level	Pattern	Image	Note
TS13: head somite	Strong		3c
TS13: embryo	Present	Regionally restricted	3c	The pattern of IGF-II hybridization was very similar to that described for embryonic day 8.0 (Theiler stage 12).

Structure	Level	Pattern	Image	Note
TS12: allantois	Strong		3d
TS12: ectoplacental cone	Weak		3d
TS12: blood island	Absent		3d

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		3e	Labelling appeared in the dorsal, lateral and ventral walls of the foregut.
TS13: embryo	Present	Regionally restricted	3e	The pattern of IGF-II hybridization was very similar to that described for embryonic day 8.0 (Theiler stage 12).

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted	3f	The pattern of IGF-II hybridization was very similar to that described for embryonic day 8.0 (Theiler stage 12).