Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:55780
Borycki AG, et al., Sonic hedgehog controls epaxial muscle determination through Myf5 activation. Development. 1999 Sep;126(18):4053-63
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1343824
|
|
Gene symbol:
|
Myf5
|
|
Gene name:
|
myogenic factor 5
|
|
Probe:
|
Myf5 probe5
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
1A,B:
embryonic day 9.75
(more  )
Specimen
1A,B:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.75
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: trunk somite |
Present |
Regionally restricted |
1B
,
1A
|
Expression is in the dorso-medial compartment specifying the progenitor cells of the epaxial muscles.
|
Specimen
2A:
embryonic day 9.75
(more )
Specimen
2A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.75
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: trunk somite |
Present |
Regionally restricted |
2A
|
Expression is in the dorso-medial compartment specifying the progenitor cells of the epaxial muscles.
|
Specimen
1C,D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 9.75
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: trunk somite |
Present |
Regionally restricted |
1C
,
1D
|
Expression is normal in the hypaxial domain, but is absent from the medial epaxial domain.
|
Specimen
2B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 9.75
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: trunk somite |
Present |
Regionally restricted |
2B
|
Expression is absent in the medial epaxial domain, although it is present elsewhere.
|
Specimen
1E,F:
embryonic day 10.5
(more )
Specimen
1E,F:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk somite |
Present |
Regionally restricted |
1F
,
1E
|
Expression is in the ventrolateral domain where hypaxial muscle progenitor cells arise.
|
Specimen
2C:
embryonic day 10.5
(more )
Specimen
2C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk somite |
Present |
Regionally restricted |
2C
|
Expression is in the ventrolateral domain where hypaxial muscle progenitor cells arise.
|
Specimen
1G,H:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk dermomyotome |
Present |
Regionally restricted |
1H
,
1G
|
There is no expression in the epaxial domain, but there is expression in the hypaxial domain.
|
Specimen
2D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk dermomyotome |
Present |
Regionally restricted |
2D
|
There is no expression in the epaxial domain, but there is expression in the hypaxial domain.
|
Specimen
2I:
embryonic day 10.5
(more )
Specimen
2I:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: tail somite |
Present |
Regionally restricted |
2I
|
Expression is in the epaxial and hypaxial domains of the newly formed somites.
|
Specimen
2J:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: tail somite |
Present |
Regionally restricted |
2J
|
In mutants, hypaxial expression expands into the entire somite.
|
|
Analysis Tools