Probe:	Ngn-2 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		7A
TS15: central nervous system	Present	Regionally restricted	7A	Expression is seen in two main longitudinal domains: one spanning the dorsal neural tube with a rostral limit corresponding to the boundary between r6 and r7, and the other throughout the ventral neural tube from the midbrain.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	7B	Expression is seen in two main longitudinal domains: one spanning the dorsal neural tube with a rostral limit corresponding to the boundary between r6 and r7, and the other throughout the ventral neural tube from the midbrain.

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Regionally restricted	7C	Both expression domains have significantly increased; the dorsal one extends to the r1/r2 boundary; in addition the gene is expressed more dorsally in r1 and in an intermediary stripe spanning r2,r3, and r4.

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		7D	Expression is similar to that in the wild-type.
TS15: central nervous system	Present	Regionally restricted	7D	Expression in the ventral hindbrain is markedly decreased, while it is less affected dorsally.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	7E	Expression in the ventral hindbrain is markedly decreased, while it is less affected dorsally.

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Regionally restricted	7F	Mutants exhibit a globally decreased activation, which is more pronounced from r4 to r7; no expression is observed dorsally in r1.