Probe:	Dlx-5 probe2
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Strong	Regionally restricted	1A	Expression is in the anterior neural ridge.

Structure	Level	Pattern	Image	Note
TS12: anterior pro-rhombomere neural fold	Present		1B
TS12: future hindbrain neural fold	Present		1B
TS12: future midbrain neural fold	Present		1B
TS12: future prosencephalon neural fold	Present		1B
TS12: future spinal cord neural fold	Present		1B
TS12: otic placode	Present		1B
TS12: posterior pro-rhombomere neural fold	Present		1B
TS12: embryo ectoderm	Present	Regionally restricted	1B	Expression is in the anterior cephalic ectoderm.

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Present		1C
TS13: otic placode	Present		1C
TS13: embryo ectoderm	Present	Regionally restricted	1C	Expression is in the anterior cephalic ectoderm.
TS13: future spinal cord neural fold	Present	Regionally restricted	1C	Expression is in the posterior neural fold.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Weak	Regionally restricted	1D	Expression is anterior, bordering the anterior neuropore.
TS13: embryo ectoderm	Present	Regionally restricted	1D	Expression is in the anterior cephalic ectoderm.

Structure	Level	Pattern	Image	Note
TS13: embryo ectoderm	Present	Regionally restricted	1E	Expression is in the anterior cephalic ectoderm.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch ectoderm	Present		1F
TS14: 1st branchial arch mesenchyme	Present		1F
TS14: caudal neuropore	Present		1F
TS14: neural tube roof plate	Present		1F
TS14: otic pit	Present		1F
TS14: embryo ectoderm	Present		1F	Expression is in the anterior cephalic ectoderm.

Structure	Level	Pattern	Image	Note
TS14: otic pit epithelium	Present	Graded	1G	Expression is strongest at the dorsal margin.
TS14: 1st branchial arch ectoderm	Present	Regionally restricted	1G	Expression is distal.
TS14: 1st branchial arch mesenchyme	Present	Regionally restricted	1G	Expression is distal.
TS14: neural tube roof plate	Present	Regionally restricted	1G	The section taken at this level shows that there is no expression but in other sections at different levels there is expression.

Structure	Level	Pattern	Image	Note
TS14: neural tube roof plate	Present		1H	This section is caudal to the one shown in 1G.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	1I	Expression is in the ectomesenchyme. The authors uses otic pit.
TS17: 1st branchial arch	Present	Regionally restricted	1I	Expression is in the ectomesenchyme.
TS17: 2nd branchial arch	Present	Regionally restricted	1I	Expression is in the ectomesenchyme.
TS17: 3rd branchial arch	Present	Regionally restricted	1I	Expression is in the ectomesenchyme.
TS17: 4th branchial arch	Present	Regionally restricted	1I	Expression is in the ectomesenchyme.
TS17: frontal process ectoderm	Present		1I
TS17: latero-nasal process ectoderm	Present		1I