Gene Expression Data
Assay Details
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Assay
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Reference:
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J:46971
Loomis CA, et al., Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development. 1998 Mar;125(6):1137-48
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1339621
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Gene symbol:
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Wnt7a
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Gene name:
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wingless-type MMTV integration site family, member 7A
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Probe:
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Wnt7a cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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All the specimens were discussed in the paper as in limb bud stages. Some assay results are only annotated to forelimb buds, but they could also be annotated to the same limb bud stage hindlimbs of a later embryonic date.
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Results
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Specimen
1G:
embryonic day 9.5
(more  )
Note: Limb-bud stage (stage 1).
Specimen
1G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 1).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud ectoderm |
Present |
Regionally restricted |
1G
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Expression is in the distal and dorsal ectoderm covering 2/3 of the limb.
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Specimen
1H:
embryonic day 9.5
(more )
Note: Limb-bud stage (stage 1).
Specimen
1H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Limb-bud stage (stage 1).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud ectoderm |
Present |
Regionally restricted |
1H
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Expression is in the distal and dorsal ectoderm covering 2/3 of the limb.
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Specimen
1I:
embryonic day 10.5
(more )
Note: Limb-bud stage (stage 3).
Specimen
1I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Limb-bud stage (stage 3).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: limb ectoderm |
Present |
Regionally restricted |
1I
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Expression is restricted to the dorsal surface.
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Specimen
2A:
embryonic day 9.5-10.5
(more )
Note: Limb-bud stage (stage 1/2).
Specimen
2A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5-10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 1/2).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: limb ectoderm |
Present |
Regionally restricted |
2A
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Expression is restricted to the dorsal surface.
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Specimen
2B:
embryonic day 9.5-10.5; En1tm1Alj/ En1tm1Alj
(more )
Note: Limb-bud stage (stage 1/2).
Specimen
2B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 9.5-10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 1/2).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: limb ectoderm |
Present |
Regionally restricted |
2B
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Mutant embryos show ectopic ventral expression, and central clearing of the expression from the ventrodistal ectoderm.
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Specimen
2E:
embryonic day 10.5
(more )
Note: Limb-bud stage (stage 3/4).
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3/4).
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Specimen
2F:
embryonic day 10.5; En1tm1Alj/ En1tm1Alj
(more )
Note: Limb-bud stage (stage 3/4).
Specimen
2F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3/4).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud ectoderm |
Present |
Regionally restricted |
2F
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Mutant embryos show ectopic ventral expression, and central clearing of the expression from the ventrodistal ectoderm.
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Specimen
Not shown:
embryonic day 9.0; En1tm1Alj/ En1tm1Alj
(more )
Note: Limb-bud stage (stage 0.5).
Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 0.5).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: forelimb bud ectoderm |
Present |
Regionally restricted |
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Mutant embryos show ectopic ventral expression.
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