Probe:	Myf5 probe2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: myotome	Present	Regionally restricted	1A , 1C	Expression is in both dorsal and ventral myotome regions including the somitic bud.
TS17: somite	Present	Regionally restricted	1A , 1C	Expression is in both dorsal and ventral myotome regions including the somitic bud.

Structure	Level	Pattern	Image	Note
TS17: myotome	Present	Regionally restricted	1G	Expression is in both dorsal and ventral myotome regions including the somitic bud.
TS17: somite	Present	Regionally restricted	1G	Expression is in both dorsal and ventral myotome regions including the somitic bud.

Structure	Level	Pattern	Image	Note
TS17: myotome	Present	Regionally restricted	1I	Expression is in both dorsal and ventral myotome regions including the somitic bud.
TS17: somite	Present	Regionally restricted	1I	Expression is in both dorsal and ventral myotome regions including the somitic bud.

Structure	Level	Pattern	Image	Note
TS17: somite 12	Present	Regionally restricted	1D , 1B	Expression is strongly reduced in the anterodorsal and posterodorsal regions of each somite, resulting in a flame-like appearance of the remaining dorsal myotome.
TS17: somite 13	Present	Regionally restricted	1D , 1B	Expression is strongly reduced in the anterodorsal and posterodorsal regions of each somite, resulting in a flame-like appearance of the remaining dorsal myotome.
TS17: somite 14	Present	Regionally restricted	1D , 1B	Expression is strongly reduced in the anterodorsal and posterodorsal regions of each somite, resulting in a flame-like appearance of the remaining dorsal myotome.
TS17: somite 15	Present	Regionally restricted	1D , 1B	Expression is strongly reduced in the anterodorsal and posterodorsal regions of each somite, resulting in a flame-like appearance of the remaining dorsal myotome.
TS17: somite 16	Present	Regionally restricted	1D , 1B	Expression is strongly reduced in the anterodorsal and posterodorsal regions of each somite, resulting in a flame-like appearance of the remaining dorsal myotome.
TS17: somite 17	Present	Regionally restricted	1D , 1B	Expression is strongly reduced in the anterodorsal and posterodorsal regions of each somite, resulting in a flame-like appearance of the remaining dorsal myotome.
TS17: myotome	Present	Regionally restricted	1B , 1D	In lower thoracic and lumbar axial regions almost no expression could be detected in dorsal myotome regions. Expression in ventral myotome regions, appeared normal. In cervical regions, the cap-like dorsal-most expression observed is missing.

Structure	Level	Pattern	Image	Note
TS17: myotome	Present	Regionally restricted	1H	Defects noted in myotome formation were detectable within the same axial regions as the abnormal Wnt3a expression in the neural tube.

Structure	Level	Pattern	Image	Note
TS17: myotome	Present	Regionally restricted	1K	Defects noted in myotome formation were detectable within the same axial regions as the abnormal Wnt3a expression in the neural tube.

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle	Weak

Structure	Level	Pattern	Image	Note
TS19: body wall mesenchyme	Weak	Regionally restricted	3G	Expression is in the developing muscles.

Structure	Level	Pattern	Image	Note
TS19: body wall mesenchyme	Weak	Regionally restricted	3H	In mutants, expression shows that ventral muscle anlagen is not impaired, but dorsal muscle anlagen is severely reduced.

Structure	Level	Pattern	Image	Note
TS21: skeletal muscle	Present	Regionally restricted	3I	In cross-sections of the upper thoracic regions, expression was detected in the developing musculature.

Structure	Level	Pattern	Image	Note
TS21: skeletal muscle	Present	Regionally restricted	3K	In cross-sections of upper thoracic regions from mutant embryos, remnants of dorsal muscle could be observed.
TS21: trunk mesenchyme	Present	Regionally restricted	3K	Patches of expression were detected in the loose dorsal mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: skeletal muscle	Present	Regionally restricted	3L	In cross-sections of the lower thoracic regions, expression was detected in the developing musculature.

Structure	Level	Pattern	Image	Note
TS21: skeletal muscle	Present	Regionally restricted	3M	In cross-sections of lower thoracic regions from mutant embryos, there was almost no dorsal muscle.
TS21: trunk mesenchyme	Present	Regionally restricted	3M	Patches of expression were detected in the loose dorsal mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: myotome	Present	Regionally restricted	5E	In advanced somites, expression is in a triangular shaped pattern of positive cells in the dorsal myotome.
TS15: somite	Present	Regionally restricted	5E	In newly generated somites, expression is in a band of cells in the dorsomedial lip.

Structure	Level	Pattern	Image	Note
TS15: myotome	Present	Regionally restricted	5F	In mutant embryos, only a central domain of expressing cells is seen in the dorsal myotome, such that large gaps in expression are apparent. The dorsomedial expression is missing.
TS15: somite	Present	Regionally restricted	5F	In mutant embryos, only a central domain of expressing cells is seen in the dorsal myotome, such that large gaps in expression are apparent. The dorsomedial expression is missing.

Structure	Level	Pattern	Image	Note
TS15: myotome	Present		5G

Structure	Level	Pattern	Image	Note
TS15: myotome	Present	Regionally restricted	5H	Aberrant expression was visible in the developing dorsal myotomes along the same axial regions where the neural tube abnormalities occurred.