Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:54638
Henderson DJ, et al., Rib truncations and fusions in the Sp2H mouse reveal a role for Pax3 in specification of the ventro-lateral and posterior parts of the somite. Dev Biol. 1999 May 1;209(1):143-58
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:1336589
|
Gene symbol:
|
Myf5
|
Gene name:
|
myogenic factor 5
|
Probe:
|
Myf5 probe3
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Beta-galactosidase
|
|
Results
|
Specimen
4.F wt:
embryonic day 10.5
(more )
Specimen
4.F wt:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4.F Sp-2H:
(close )
Genetic Background:
|
involves: C57BL/6
|
Mutant Allele(s):
|
Pax3Sp-2H/Pax3Sp-2H
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk somite |
Present |
Regionally restricted |
4F right
|
Expression domain is truncated ventrally in mutant embryos.
|
Specimen
4.G:
embryonic day 10.5
(more )
Note: Embryos were sectioned in 0.45% gelatine, 27% albumin, 18% glucose in PBS which was solidified by the addition of 1/10 volume of 30% glutaraldehyde.
Specimen
4.G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Embryos were sectioned in 0.45% gelatine, 27% albumin, 18% glucose in PBS which was solidified by the addition of 1/10 volume of 30% glutaraldehyde.
|
Specimen
4.H:
embryonic day 10.5; Pax3Sp-2H/ Pax3Sp-2H
(more )
Note: Embryos were sectioned in 0.45% gelatine, 27% albumin, 18% glucose in PBS which was solidified by the addition of 1/10 volume of 30% glutaraldehyde.
Specimen
4.H:
(close )
Genetic Background:
|
involves: C57BL/6
|
Mutant Allele(s):
|
Pax3Sp-2H/Pax3Sp-2H
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Embryos were sectioned in 0.45% gelatine, 27% albumin, 18% glucose in PBS which was solidified by the addition of 1/10 volume of 30% glutaraldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk dermomyotome |
Present |
Regionally restricted |
4H
|
Expression is domain is truncated ventrally in the mutant.
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: C57BL/6
|
Mutant Allele(s):
|
Pax3Sp-2H/Pax3Sp-2H
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
Not Specified
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: trunk dermomyotome |
Present |
Regionally restricted |
|
Authors report ventral truncation of the expression domain; domain appears to be slightly expanded mediolaterally. No ectopic expression outside of the myotome.
|
|