Probe:	Fgf8 cDNA13
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS8: extraembryonic visceral endoderm	Present		3C
TS8: extraembryonic component	Present		3C

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	5D	Expression is in a sharp-narrow band in the region of the midbrain-hindbrain junction (isthmus). This does not include cells of the ventral midline. Expression is immediately caudal to Wnt1 expression.
TS15: future midbrain floor plate	Present	Regionally restricted	5D	Expression is in a sharp-narrow band in the region of the midbrain-hindbrain junction (isthmus). This does not include cells of the ventral midline. Expression is immediately caudal to Wnt1 expression.
TS15: future midbrain lateral wall	Present	Regionally restricted	5D	Expression is in a sharp-narrow band in the region of the midbrain-hindbrain junction (isthmus). This does not include cells of the ventral midline. Expression is immediately caudal to Wnt1 expression.
TS15: future midbrain roof plate	Present	Regionally restricted	5D	Expression is in a sharp-narrow band in the region of the midbrain-hindbrain junction (isthmus). This does not include cells of the ventral midline. Expression is immediately caudal to Wnt1 expression.
TS15: telencephalon floor plate	Present	Regionally restricted	5D	Expression is in the commisural plate.
TS15: telencephalon lateral wall	Present	Regionally restricted	5D	Expression is in the commisural plate.
TS15: telencephalon roof plate	Present	Regionally restricted	5D	Expression is in the commisural plate.
TS15: diencephalon gland	Present	Regionally restricted	5D	Expression is in the infundibular region of the hypothalamus.
TS15: future neurohypophysis	Present	Regionally restricted	5D	Expression is in the posterior pituitary with a sharp caudal boundary at the mammillary area.
TS15: future forebrain	Present	Regionally restricted	5D	Expression is in the alar plate of the ventral thalamus and extends lateral to the dorsal midline just rostral to the zona limitans.

Structure	Level	Pattern	Image	Note
TS15: optic recess	Present	Regionally restricted	5F	Expression is in a ventral zone.
TS15: optic stalk	Present	Regionally restricted	5F	Expression is in a ventral zone.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	5H	Expression is at the midbrain-hindbrain junction (isthmus).
TS20: midbrain	Present	Regionally restricted	5H	Expression is at the midbrain-hindbrain junction (isthmus).
TS20: diencephalon roof plate	Present	Regionally restricted	5H	Expression is rostral to the developing pineal gland.
TS20: telencephalon	Present	Regionally restricted	5H	Expression is in the commisural plate.
TS20: neurohypophysis infundibulum	Present	Regionally restricted	5H	Expression is in the infundibular ventral midline and posterior pituitary.
TS20: pituitary gland	Present	Regionally restricted	5H	Expression is in the infundibular ventral midline and posterior pituitary.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	5I	Expression is at the midbrain-hindbrain junction (isthmus).
TS20: midbrain	Present	Regionally restricted	5I	Expression is at the midbrain-hindbrain junction (isthmus).
TS20: optic chiasma	Present		5I	Expression is also in the optic nerve.
TS20: optic stalk	Present		5I	Expression is also in the optic nerve.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Present	Regionally restricted	7A	Expression is in the ventral ectoderm.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud apical ectodermal ridge	Present		7B
TS16: forelimb bud ectoderm	Present	Regionally restricted	7B	There is no longer expression in the ventral ectoderm (although it is still present in the apical ectodermal ridge).