Probe:	Fgf8 cDNA13
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present	Regionally restricted	3B	Expression is in the ectoderm on one side of the embryo. The proximal boundary of expression coincides with the embryonic-extraembryonic junction and extends around approximately half the circumfrence of the epiblast.
TS8: extraembryonic component	Absent		3B
TS28: decidua	Absent		3B

Structure	Level	Pattern	Image	Note
TS9: primitive streak	Present		3D

Structure	Level	Pattern	Image	Note
TS11: allantois	Strong	Regionally restricted	4A	Expression is in cells at the base of the allantois.
TS11: primitive streak	Present	Graded	4A	Expression is stronger in the proximal streak than in the distal streak. Expression is reduced as cells pass through and emerge from the streak.
TS11: embryo ectoderm	Present	Regionally restricted	4A	Expression is in the cells immediately lateral to the streak.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	4B , 4F	Expression is in the streak and the ectoderm adjacent to it with a sharp boundary at the node. There is also expression in the mesodermal cells emerging from the streak.
TS11: embryo mesoderm	Present	Regionally restricted	4B , 4F	Expression is in the streak and the ectoderm adjacent to it with a sharp boundary at the node. There is also expression in the mesodermal cells emerging from the streak.
TS11: node	Present	Regionally restricted	4B , 4F	Expression is in the streak and the ectoderm adjacent to it with a sharp boundary at the node. There is also expression in the mesodermal cells emerging from the streak.
TS11: primitive streak	Present	Regionally restricted	4B , 4F	Expression is in the streak and the ectoderm adjacent to it with a sharp boundary at the node. There is also expression in the mesodermal cells emerging from the streak.
TS11: intraembryonic coelom	Present		4F

Structure	Level	Pattern	Image	Note
TS12: head somite	Absent		4C , 4G
TS12: trunk somite	Absent		4C , 4G
TS12: trunk unsegmented mesenchyme	Absent		4C , 4G
TS12: head paraxial mesenchyme	Present		4C , 4G	Expression is in the cells leaving the streak.
TS12: trunk paraxial mesenchyme	Present		4C , 4G	Expression is in the cells leaving the streak.
TS12: embryo mesenchyme	Present	Regionally restricted	4G , 4C	Expression is in the primitve streak and in the mesoderm emerging from the streak.
TS12: primitive streak	Present	Regionally restricted	4G , 4C	Expression is in the primitve streak and in the mesoderm emerging from the streak.
TS12: cardiovascular system	Present	Regionally restricted	4G	Expression is in the heart and associated vessels.

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon floor plate	Present	Regionally restricted	5A	Expression is at the rostral end.
TS12: anterior pro-rhombomere floor plate	Present	Regionally restricted	5A	Expression is at the midbrain-hindbrain junction.
TS12: future midbrain floor plate	Present	Regionally restricted	5A	Expression is at the midbrain-hindbrain junction.
TS12: arterial system	Absent		5A
TS12: heart	Absent		5A
TS12: venous system	Absent		5A

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon floor plate	Present		5B	Expression is in a bilaterally symmetric group of cells at the rostral end of the neural plate.
TS13: anterior pro-rhombomere floor plate	Present		5B	Expression is at the midbrain-hindbrain border.
TS13: future midbrain floor plate	Present		5B	Expression is at the midbrain-hindbrain border.
TS13: arterial system	Absent		5B
TS13: heart	Absent		5B
TS13: venous system	Absent		5B

Structure	Level	Pattern	Image	Note
TS15: cardiovascular system	Absent		5C
TS15: diencephalon floor plate	Present		5C
TS15: tail	Present		5C
TS15: telencephalon floor plate	Present		5C
TS15: future hindbrain	Present	Regionally restricted	5C	Expression is at the midbrain-hindbrain border.
TS15: future midbrain	Present	Regionally restricted	5C	Expression is at the midbrain-hindbrain border.

Structure	Level	Pattern	Image	Note
TS15: olfactory placode	Present	Regionally restricted	6D	Expression is in the surface ectoderm.
TS15: 1st branchial arch mandibular component ectoderm	Present		6D
TS15: 1st branchial arch maxillary component ectoderm	Present		6D

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component ectoderm	Present		6E
TS17: 1st branchial arch maxillary component ectoderm	Present		6E
TS17: 1st branchial groove	Present		6E
TS17: 2nd branchial groove	Present		6E
TS17: 3rd branchial groove	Present		6E
TS17: 4th branchial groove	Present		6E
TS17: nasal pit epithelium	Present		6E
TS17: diencephalon floor plate	Present	Regionally restricted	6E	Expression is in the commissural plate.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Strong	Regionally restricted	7C
TS17: hindlimb bud apical ectodermal ridge	Strong	Regionally restricted	7C

Structure	Level	Pattern	Image	Note
TS14: trunk somite	Present	Regionally restricted	4D	Expression is in the lateral region of the myotome.

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Present	Regionally restricted	4E	Expression is in the lateral region of the myotome.

Structure	Level	Pattern	Image	Note
TS11: intraembryonic coelom	Present		4H
TS11: primitive streak	Present		4H

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum endoderm	Present		4I
TS12: foregut diverticulum mesenchyme	Present		4I
TS12: intraembryonic coelom	Present		4I

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum endoderm	Present		6B
TS12: foregut diverticulum mesenchyme	Present		6B
TS12: embryo ectoderm	Present	Regionally restricted	6B	Expression is in the surface ectoderm overlying the foregut.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial groove ectoderm	Present		6C
TS15: 1st branchial pouch endoderm	Present		6C
TS15: 2nd branchial groove ectoderm	Present		6C
TS15: 2nd branchial pouch endoderm	Present		6C
TS15: 3rd branchial groove ectoderm	Present		6C
TS15: 3rd branchial pouch endoderm	Present		6C
TS15: 1st branchial arch	Present	Regionally restricted	6C	Expression is in the surface ectoderm.