Probe:	Pax3 probe2
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		6A
TS15: 1st branchial arch	Present	Regionally restricted	6A	Pax3 was expressed in streams of neural crest cells that could be seen emerging from rhombomeres 2 and 4, entering branchial arches 1 and 2 respectively. Streams of cells could also be seen emerging from the neural tube caudal to the otic vesicle.
TS15: 2nd branchial arch	Present	Regionally restricted	6A	Pax3 was expressed in streams of neural crest cells that could be seen emerging from rhombomeres 2 and 4, entering branchial arches 1 and 2 respectively. Streams of cells could also be seen emerging from the neural tube caudal to the otic vesicle.
TS15: embryo	Present	Regionally restricted	6A	Pax3 was expressed in streams of neural crest cells that could be seen emerging from rhombomeres 2 and 4, entering branchial arches 1 and 2 respectively. Streams of cells could also be seen emerging from the neural tube caudal to the otic vesicle.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		6B
TS15: 1st branchial arch	Present	Regionally restricted	6B	Expression in this mutant is the same as in wild-type embryos.
TS15: 2nd branchial arch	Present	Regionally restricted	6B	Expression in this mutant is the same as in wild-type embryos.
TS15: embryo	Present	Regionally restricted	6B	Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		7A
TS17: 3rd branchial arch	Present	Regionally restricted	7A , 7C	Streams of cells were detected passing though the developing third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.
TS17: 4th branchial arch	Present	Regionally restricted	7A , 7C	Streams of cells were detected passing though the developing third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.
TS17: 6th branchial arch	Present	Regionally restricted	7A , 7C	Streams of cells were detected passing though the developing third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.
TS17: embryo	Present	Regionally restricted	7A , 7C	Streams of cells were detected passing though the developing third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.
TS17: outflow tract	Present	Regionally restricted	7A , 7C	Streams of cells were detected passing though the developing third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		7B
TS17: 3rd branchial arch	Weak	Regionally restricted	7B	Mutant embryos either failed to exhibit the stream of Pax3-positive cells or showed marked reduction in the number of expressing cells passing through the third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.
TS17: 4th branchial arch	Weak	Regionally restricted	7B	Mutant embryos either failed to exhibit the stream of Pax3-positive cells or showed marked reduction in the number of expressing cells passing through the third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.
TS17: 6th branchial arch	Weak	Regionally restricted	7B	Mutant embryos either failed to exhibit the stream of Pax3-positive cells or showed marked reduction in the number of expressing cells passing through the third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.
TS17: embryo	Weak	Regionally restricted	7B	Mutant embryos either failed to exhibit the stream of Pax3-positive cells or showed marked reduction in the number of expressing cells passing through the third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.
TS17: outflow tract	Weak	Regionally restricted	7B	Mutant embryos either failed to exhibit the stream of Pax3-positive cells or showed marked reduction in the number of expressing cells passing through the third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.

Structure	Level	Pattern	Image	Note
TS17: 4th branchial arch	Present	Regionally restricted	8B/C	Pax-3 positive cels were observed in control embryos within pharyngeal arches 4 and 6, at the junction of the aortic sac.
TS17: 6th branchial arch	Present	Regionally restricted	8B/C	Pax-3 positive cels were observed in control embryos within pharyngeal arches 4 and 6, at the junction of the aortic sac.

Structure	Level	Pattern	Image	Note
TS17: 4th branchial arch	Absent		8E/F
TS17: 6th branchial arch	Absent		8E/F

Structure	Level	Pattern	Image	Note
TS19: heart	Absent