Probe:	pPrd3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS10: embryo endoderm	Present	Regionally restricted	5A , 5B	Expression is at the cephalic end of the embryo.
TS10: embryo mesoderm	Present	Regionally restricted	5A , 5B	Expression is in the prechordal plate at the cephalic end of the embryo.

Structure	Level	Pattern	Image	Note
TS10: embryo endoderm	Present		5D , 5C	Expression is at the cephalic end of the embryo.
TS10: embryo mesoderm	Present	Regionally restricted	5C , 5D	Expression is in the prechordal plate at the cephalic end of the embryo.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Regionally restricted	5E/F	Expression is in the prechordal plate that will become the cephalic neural plate.

Structure	Level	Pattern	Image	Note
TS9: embryo endoderm	Present	Regionally restricted	6A	Expression is in the endodermal layer that is directly across from the ectodermal region that expresses T.

Structure	Level	Pattern	Image	Note
TS10: embryo endoderm	Present		6C
TS10: embryo mesoderm	Present	Regionally restricted	6C	Expression is in the prechordal plate precursor.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	6E	There is no expression in the most distal sections.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	6G	Expression in more proximal sections is in prechordal plate at the boundary of the notochordal plate. The notochordal plate is expressing T.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	6I	Expression in the most proximal sections is in the prechordal plate and the cephalic neural plate.
TS10: neural ectoderm	Present	Regionally restricted	6I	Expression in the most proximal sections is in the prechordal plate and the cephalic neural plate.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Absent		7A/B	Note the lack of expression in mesodermal cells on either side of the midline.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Regionally restricted	7C/D	Expression is in the cephalic neurectoderm.
TS11: embryo mesoderm	Absent		7C/D	No expression in the mesendoderm of the prechordal plate or the head mesoderm.
TS11: mesendoderm	Absent		7C/D	No expression in the mesendoderm of the prechordal plate or the head mesoderm.

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	8A/B	Expression is restricted to the cephalic neurectoderm.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present		8C/D
TS13: embryo ectoderm	Present	Regionally restricted	8C/D	Expression is in the oral ectoderm and in a small region of the rostral foregut endoderm that is in contact with the Hsex1-expressing oral ectoderm.
TS13: foregut diverticulum endoderm	Present	Regionally restricted	8C/D	Expression is in the oral ectoderm and in a small region of the rostral foregut endoderm that is in contact with the Hsex1-expressing oral ectoderm.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	8E/F	Expression is resticted to the most rostral neurectoderm.

Structure	Level	Pattern	Image	Note
TS15: Rathke's pouch	Present		9A/B

Structure	Level	Pattern	Image	Note
TS19: Rathke's pouch	Strong		9C/D

Structure	Level	Pattern	Image	Note
TS23: pituitary gland	Absent		9E/F

Structure	Level	Pattern	Image	Note
TS20: pituitary gland	Present	Regionally restricted	10B , 10A	Expression is progressively downregulated in a ventral to dorsal direction.

Structure	Level	Pattern	Image	Note
TS21: remnant of Rathke's pouch	Present	Regionally restricted	10C	Expression is not detected in the ventral region of the pouch, but is detected in the dorsal region of the pouch.

Structure	Level	Pattern	Image	Note
TS22: remnant of Rathke's pouch	Present	Regionally restricted	10E	Expression only persists in the dorsal region that is in contact with the floor of the diencephalon and posterior lobe.