Twist1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:53294 Solloway MJ, et al., Early embryonic lethality in Bmp5;Bmp7 double mutant mice suggests functional redundancy within the 60A subgroup. Development. 1999 Apr;126(8):1753-68
Assay type:	RNA in situ
MGI Accession ID:	MGI:1333106
Gene symbol:	Twist1
Gene name:	twist basic helix-loop-helix transcription factor 1

Probe:	M-twist cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

6A upper ©

Specimen 6A upper: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch mesenchyme	Present		6A upper
TS13: head mesenchyme	Present		6A upper
TS13: extraembryonic component	Present	Regionally restricted	6A upper	Expression is in the distal allantoic mesenchyme.

6A lower ©

Specimen 6A lower: embryonic day 8.5; Bmp5^se/Bmp5^se, Bmp7^tm1Rob/Bmp7^tm1Rob (more

)

Structure	Level	Image	Note
TS13: 1st branchial arch mesenchyme	Present	6A lower	There is a decrease in the domain of expression in double-mutant embryos.
TS13: head mesenchyme	Present	6A lower	There is a decrease in the domain of expression in double-mutant embryos.
TS13: extraembryonic component	Present	6A lower	There is a decrease in the domain of expression in double-mutant embryos

6B ©

Specimen 6B: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present		6B
TS15: mesenchyme derived from somatopleure	Present		6B
TS15: trunk paraxial mesenchyme	Present		6B
TS15: extraembryonic component	Present	Regionally restricted	6B	Expression is in the mesenchyme of the allantois.

6C ©

Specimen 6C: embryonic day 9.5; Bmp5^se/Bmp5^se, Bmp7^tm1Rob/Bmp7^tm1Rob (more

)

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present		6C	The domains of expression are reduced compared to wild-type embryos.
TS15: mesenchyme derived from somatopleure	Present		6C	The domains of expression are reduced compared to wild-type embryos.
TS15: trunk paraxial mesenchyme	Present		6C	The domains of expression are reduced compared to wild-type embryos.
TS15: allantois	Weak	Regionally restricted	6C	Expression is in the mesenchyme of the allantois. The expression level is reduced compared to wild-type.

6D ©

Specimen 6D: embryonic day 9.5; Bmp5^se/Bmp5^se, Bmp7^tm1Rob/Bmp7^tm1Rob (more

)

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present		6D	The domains of expression are reduced compared to wild-type embryos.
TS15: mesenchyme derived from somatopleure	Present		6D	The domains of expression are reduced compared to wild-type embryos.
TS15: trunk paraxial mesenchyme	Present		6D	The domains of expression are reduced compared to wild-type embryos.
TS15: allantois	Weak	Regionally restricted	6D	Expression is in the mesenchyme of the allantois. The expression level is reduced compared to wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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