Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:42110
Wassarman KM, et al., Specification of the anterior hindbrain and establishment of a normal mid/hindbrain organizer is dependent on Gbx2 gene function. Development. 1997 Aug;124(15):2923-34
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1331431
|
|
Gene symbol:
|
Fgf8
|
|
Gene name:
|
fibroblast growth factor 8
|
|
Probe:
|
Fgf8 cDNA13
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
3I:
embryonic day 9.5
(more  )
Specimen
3I:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
3I
|
Expression is noted in a sharp transverse stripe at the anterior boundary of the hindbrain.
|
| TS15: future forebrain |
Present |
Regionally restricted |
3I
|
Expression is noted in a sharp transverse stripe at the anterior boundary of the hindbrain.
|
| TS15: future hindbrain |
Present |
Regionally restricted |
3I
|
Expression is noted in a sharp transverse stripe at the anterior boundary of the hindbrain.
|
| TS15: future midbrain |
Present |
Regionally restricted |
3I
|
Expression is noted in a sharp transverse stripe at the anterior boundary of the hindbrain.
|
Specimen
3J:
(close )
|
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2J) or (involves: 129S1/Sv * 129X1/SvJ * FVB/N)
|
|
Mutant Allele(s):
|
Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
3J
|
Expression in the mutant is in an expanded diffuse band extending caudally from the end of the midbrain. Expression in the forebrain and other regions of the embryo is normal.
|
| TS15: future forebrain |
Present |
Regionally restricted |
3J
|
Expression in the mutant is in an expanded diffuse band extending caudally from the end of the midbrain. Expression in the forebrain and other regions of the embryo is normal.
|
| TS15: future hindbrain |
Present |
Regionally restricted |
3J
|
Expression in the mutant is in an expanded diffuse band extending caudally from the end of the midbrain. Expression in the forebrain and other regions of the embryo is normal.
|
| TS15: future midbrain |
Present |
Regionally restricted |
3J
|
Expression in the mutant is in an expanded diffuse band extending caudally from the end of the midbrain. Expression in the forebrain and other regions of the embryo is normal.
|
Specimen
4G:
embryonic day 10.5
(more )
Specimen
4G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Present |
Regionally restricted |
4G
|
Expression is noted in a sharp transverse stripe at the anterior boundary of the hindbrain.
|
| TS17: forebrain |
Present |
Regionally restricted |
4G
|
Expression is noted in a sharp transverse stripe at the anterior boundary of the hindbrain.
|
| TS17: hindbrain |
Present |
Regionally restricted |
4G
|
Expression is noted in a sharp transverse stripe at the anterior boundary of the hindbrain.
|
| TS17: midbrain |
Present |
Regionally restricted |
4G
|
Expression is noted in a sharp transverse stripe at the anterior boundary of the hindbrain.
|
Specimen
4H:
(close )
|
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2J) or (involves: 129S1/Sv * 129X1/SvJ * FVB/N)
|
|
Mutant Allele(s):
|
Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Present |
Regionally restricted |
4H
|
Expression in the mutant is in an expanded diffuse band extending caudally from the end of the midbrain. Expression in the forebrain and other regions of the embryo is normal.
|
| TS17: forebrain |
Present |
Regionally restricted |
4H
|
Expression in the mutant is in an expanded diffuse band extending caudally from the end of the midbrain. Expression in the forebrain and other regions of the embryo is normal.
|
| TS17: hindbrain |
Present |
Regionally restricted |
4H
|
Expression in the mutant is in an expanded diffuse band extending caudally from the end of the midbrain. Expression in the forebrain and other regions of the embryo is normal.
|
| TS17: midbrain |
Present |
Regionally restricted |
4H
|
Expression in the mutant is in an expanded diffuse band extending caudally from the end of the midbrain. Expression in the forebrain and other regions of the embryo is normal.
|
|
Analysis Tools