Gene Expression Data
Assay Details
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Assay
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Reference:
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J:42110
Wassarman KM, et al., Specification of the anterior hindbrain and establishment of a normal mid/hindbrain organizer is dependent on Gbx2 gene function. Development. 1997 Aug;124(15):2923-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1331430
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Gene symbol:
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Hoxb1
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Gene name:
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homeobox B1
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Probe:
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probe 1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3G:
embryonic day 9.5
(more  )
Specimen
3G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3H:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2J) or (involves: 129S1/Sv * 129X1/SvJ * FVB/N)
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: rhombomere 4 |
Present |
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3H
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The expression is detected in cells near the isthmic constriction. The authors suggest that this is due to a reduction of R3 in mutant embryos.
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Specimen
4C:
embryonic day 10.5
(more )
Specimen
4C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
4D:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2J) or (involves: 129S1/Sv * 129X1/SvJ * FVB/N)
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 4 |
Present |
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4D
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The expression is detected in cells near the isthmic constriction. The authors suggest that this is due to a reduction of R3 in mutant embryos.
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Specimen
6B:
embryonic day 7.75
(more )
Specimen
6B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Present |
Regionally restricted |
6B
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The anterior boundary of expression marks the anterior end of rhombomere 4.
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| TS11: embryo |
Present |
Regionally restricted |
6B
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Expression is in all three germ layers.
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Analysis Tools