Gene Expression Data
Assay Details
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Assay
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Reference:
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J:43764
Takihara Y, et al., Targeted disruption of the mouse homologue of the Drosophila polyhomeotic gene leads to altered anteroposterior patterning and neural crest defects. Development. 1997 Oct;124(19):3673-82
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1330923
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Gene symbol:
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Hoxb3
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Gene name:
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homeobox B3
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Probe:
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Hoxb3 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5G:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Phc1tm1Os/Phc1tm1Os
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: chondrocranium |
Present |
Regionally restricted |
5G
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The anterior boundary of expression shifts from prevertebra 1 to the caudal region of the basoccipital anlage.
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| TS20: pons |
Present |
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5G
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Expression is shifted from the medulla oblongata to the pons.
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Specimen
5H:
embryonic day 12.5
(more  )
Specimen
5H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Phc1tm1Os/Phc1tm1Os
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 4 |
Present |
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6B
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The anterior expression boundary was shifted from rhombomere 5 to rhombomere 4.
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| TS17: 2nd branchial arch |
Present |
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6B
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The anterior expression boundary was shifted from pharyngeal arch 3 to pharyngeal arch 2.
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Specimen
6C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Phc1tm1Os/Phc1tm1Os
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: 2nd branchial arch |
Present |
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6C
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The anterior expression boundary was shifted from pharyngeal arch 3 to pharyngeal arch 2.
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Specimen
6D:
embryonic day 10.5
(more )
Specimen
6D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
Hoxb-3 rae28-/-:
embryonic day 10.5; Phc1tm1Os/ Phc1tm1Os
(more )
Note: These data shown in tabular form in Table 3.
Specimen
Hoxb-3 rae28-/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Phc1tm1Os/Phc1tm1Os
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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These data shown in tabular form in Table 3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere |
Present |
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5/5 embryos showed ectopic expression in the rhombomeres.
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Specimen
Hoxb-3 rae28-/-:
embryonic day 12.5; Phc1tm1Os/ Phc1tm1Os
(more )
Note: These data shown in tabular form in Table 3.
Specimen
Hoxb-3 rae28-/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Phc1tm1Os/Phc1tm1Os
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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These data shown in tabular form in Table 3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: embryo mesenchyme |
Present |
Regionally restricted |
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5/5 embryos showed ectopic expression in the paraxial mesoderm and neural components.
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| TS20: nervous system |
Present |
Regionally restricted |
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5/5 embryos showed ectopic expression in the paraxial mesoderm and neural components.
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Analysis Tools