Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:43763
Gavalas A, et al., Role of Hoxa-2 in axon pathfinding and rostral hindbrain patterning. Development. 1997 Oct;124(19):3693-702
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1330756
|
|
Gene symbol:
|
Zbtb16
|
|
Gene name:
|
zinc finger and BTB domain containing 16
|
|
Probe:
|
PLZF probe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
The scale bar in Fig. 2C is 450um.
|
|
|
Results
|
Specimen
2C:
embryonic day 10.5
(more  )
Specimen
2C:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Strong |
Regionally restricted |
2C
|
Expression is at rhombomeric boundaries.
|
Specimen
2D:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Hoxa2tm1Ipc/Hoxa2tm1Ipc
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
2D
|
Expression in the mutant shows deletion of the rhombomere 1/2 boudary and a compromise in the integrity of the rhombomere 2/3 boundary.
|
|
Analysis Tools