Gene Expression Data
Assay Details
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Assay
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Reference:
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J:52599
Mendelsohn C, et al., Stromal cells mediate retinoid-dependent functions essential for renal development. Development. 1999 Mar;126(6):1139-48
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1330551
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Gene symbol:
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Wt1
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Gene name:
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WT1 transcription factor
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Probe:
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Wt-1 genomic1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A:
embryonic day 12.0
(more  )
Note: The plane of section is transverse.
Specimen
5A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section is transverse.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mesenchyme of metanephros |
Present |
Regionally restricted |
5A
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Expression was detected in peripheral and condensing metanephric mesenchyme surrounding the ureteric bud tips.
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Raratm1Ipc/Raratm1Ipc
Rarbtm1Ipc/Rarbtm1Ipc
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section is transverse.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mesenchyme of metanephros |
Present |
Regionally restricted |
5B
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Expression was detected in peripheral and condensing metanephric mesenchyme surrounding the ureteric bud tips as in wild type.
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Specimen
6G:
embryonic day 14.0
(more )
Specimen
6G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6H:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Raratm1Ipc/Raratm1Ipc
Rarbtm1Ipc/Rarbtm1Ipc
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mesenchyme of metanephros |
Present |
Regionally restricted |
6H
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Expression was detected surrounding the ureteric bud and in a thickened layer of putative stromal cells at the periphery of the mutant kidneys.
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| TS22: maturing glomerular tuft |
Present |
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6H
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Expression was detected in the podocyte precursors in S-shaped bodies.
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Analysis Tools