Probe:	Wnt5a probe21
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Weak	Regionally restricted	1a	Low levels in mesoderm lateral to the primitive streak.
TS11: primitive streak	Present	Regionally restricted	1a	Highest levels in the mesoderm of the caudal primitive streak, diminishing to lower levels in the anterior streak.
TS11: allantois	Present		1a

Structure	Level	Pattern	Image	Note
TS14: head somite	Absent		1b
TS14: 1st branchial arch	Present		1b
TS14: trunk paraxial mesenchyme	Present	Regionally restricted	1b	Expression is in the caudal paraxial mesoderm. The rostral limit is difficult to establish since transcript levels diminish over time.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Present	Regionally restricted	1c	Transcripts are localised to the ventral limb ectoderm and in slightly later-stage embryos to the apical ectodermal ridge.
TS15: 1st branchial arch mandibular component	Present	Graded	1c	Expressed in a graded fashion in outgrowing regions of the facial primordia.
TS15: 1st branchial arch maxillary component	Present	Graded	1c	Expressed in a graded fashion in outgrowing regions of the facial primordia.

Structure	Level	Pattern	Image	Note
TS18: gut	Present	Regionally restricted	1d	Expression is in the mesenchyme of the developing gut.
TS18: limb	Present	Regionally restricted	1d	Expression is in the apical ectodermal ridge, while expression in the mesenchyme becomes graded.
TS18: gonad primordium	Present		1d

Structure	Level	Pattern	Image	Note
TS18: embryo	Present	Regionally restricted		Expression at the caudal end of the embryo in all three germ layers including hindgut endoderm.

Structure	Level	Pattern	Image	Note
TS19: limb	Present	Regionally restricted	1e	Ectodermal expression is down-regulated and graded transcripts are confined to the distal mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: limb	Present	Regionally restricted	1f	Expression is excluded from the core mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Present	Regionally restricted	1g	Expressed in the distal two thirds of the digit perichondrium.

Structure	Level	Pattern	Image	Note
TS22: radius cartilage condensation	Present	Regionally restricted		Expressed in the perichondrium of the radius and ulna.
TS22: ulna cartilage condensation	Present	Regionally restricted		Expressed in the perichondrium of the radius and ulna.

Structure	Level	Pattern	Image	Note
TS22: genital tubercle	Present	Graded	1h	Highest expression levels are distal.

Structure	Level	Pattern	Image	Note
TS20: gut	Present	Regionally restricted	1i	Expressed in mesenchyme.
TS20: embryo	Present	Graded	1i	Graded expression in the face and tongue.
TS20: tongue	Present	Graded	1i	Graded expression in the face and tongue.
TS20: axial skeleton	Present	Regionally restricted	1i	Perichondrial expression is prominent.
TS20: genital tubercle	Present	Graded	1i	Expression is highest distally.

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Regionally restricted	1j	Expression is in the perichondrium and adjacent mesenchyme cells.

Structure	Level	Pattern	Image	Note
TS21: limb	Present		7g lower

Structure	Level	Pattern	Image	Note
TS20: limb	Absent		7g upper