Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:51989
Suda Y, et al., Functional equivalency between Otx2 and Otx1 in development of the rostral head. Development. 1999 Feb;126(4):743-57
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:1328441
|
Gene symbol:
|
Six3
|
Gene name:
|
sine oculis-related homeobox 3
|
Probe:
|
Six3 probe2
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Results
|
Specimen
5M:
embryonic day 8.5
(more )
Specimen
5M:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: prosencephalon |
Present |
Regionally restricted |
5M
|
Expression is in the anterior ridge of the future forebrain.
|
Specimen
6D:
embryonic day 7.5-7.75
(more )
Specimen
6D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5-7.75
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: neural ectoderm |
Present |
Regionally restricted |
6D
|
Expression is in the rostral neural ectoderm.
|
Specimen
6F:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA
|
Mutant Allele(s):
|
Otx2tm1Sia/Otx2tm1Sia
|
Age:
|
embryonic day 7.5-7.75
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: neural ectoderm |
Absent |
|
6F
|
Six3 was used as a marker for rostral neural ectoderm in these mutant embryos.
|
|