Gene Expression Data
Assay Details
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Assay
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Reference:
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J:50310
Downs KM, et al., Vascularization in the murine allantois occurs by vasculogenesis without accompanying erythropoiesis. Development. 1998 Nov;125(22):4507-20
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1298414
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Gene symbol:
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Kdr
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Gene name:
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kinase insert domain protein receptor
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Results
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Specimen
3A:
embryonic day 7.5
(more  )
Note: Neural plate stage.
Specimen
3A:
(close  )
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Genetic Background:
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(C57BL/6 x CBA)F1
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Neural plate stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: allantois mesoderm |
Present |
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3A
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| TS11: allantois |
Present |
Regionally restricted |
3A
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flk-1 was not observed in the mesothelium of the allantois. The percentage of positive core cells was highest in the distal third of the allantoic bud, significantly lower in the basal third and intermediate in the middle third.
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Specimen
3B:
embryonic day 8.0
(more )
Note: Late headfold stage.
Specimen
3B:
(close )
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Genetic Background:
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(C57BL/6 x CBA)F1
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Age:
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embryonic day 8.0
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Late headfold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: allantois mesoderm |
Present |
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3B
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| TS11: allantois |
Present |
Regionally restricted |
3B
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flk-1 was not observed in the mesothelium of the allantois. The percentage of positive core cells was highest in the distal third of the allantoic bud, significantly lower in the basal third and intermediate in the middle third.
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Specimen
3C:
embryonic day 8.0
(more )
Note: 3.5-somite pairs.
Specimen
3C:
(close )
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Genetic Background:
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(C57BL/6 x CBA)F1
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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3.5-somite pairs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: allantois mesenchyme |
Present |
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3C
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| TS12: allantois |
Present |
Regionally restricted |
3C
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flk-1 was not observed in the mesothelium of the allantois. Expression had become abundant in the basal third of the allantois and was invariably expressed in the nascent allantoic blood vessel found at the allantoic/yolk sac junction at 4-somite pairs.
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Specimen
3F:
embryonic day 8.0
(more )
Note: 6-somite pairs.
Specimen
3F:
(close )
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Genetic Background:
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(C57BL/6 x CBA)F1
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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6-somite pairs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: allantois mesenchyme |
Present |
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3F
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| TS12: allantois |
Present |
Regionally restricted |
3F
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flk-1 was not observed in the mesothelium of the allantois. Flk-1 protein was abundant in flattened cells that formed obvious vascular channels throughout the allantois.
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Specimen
3G:
embryonic day 8.5
(more )
Note: 10-somite pairs.
Specimen
3G:
(close )
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Genetic Background:
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(C57BL/6 x CBA)F1
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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10-somite pairs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: extraembryonic component |
Present |
Regionally restricted |
3G
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The core mesoderm of the allantois contained Flk-1-positive cells. flk-1 was not observed in the mesothelium of the allantois. Flk-1 protein was abundant in flattened cells that formed obvious vascular channels throughout the allantois.
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