Antibody:	anti-RERE (H-113; sc-98415)
Detection system:	Secondary antibody coupled to Alexa Fluor 568

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Present	Widespread	1A	Broadly expressed.
TS23: rhombic lip	Absent		1A

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Present	Widespread	1B	Expression was still maintained in the entire cerebellum.
TS25: cerebellum external granule cell layer	Present	Punctate	1B	Less intense than seen in other regions of the cerebellum, and did not always counterstain with DAPI.

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present	Widespread	1C	Positive cells were seen in the entire cerebellar region.
TS27: cerebellum Purkinje cell layer	Strong		1C

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Widespread	1D	Positive cells were seen in the entire cerebellar region.
TS28: cerebellum Purkinje cell layer	Strong		1D
TS28: cerebellum internal granule cell layer	Present		1D
TS28: cerebellum molecular layer	Present		1D

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cerebellum Purkinje cell cluster	Purkinje cell	Present		2A merge , 2A RERE	Coexpressed with Nr2f2 in a subset of Purkinje cells. Not detected in all Nr2f2-expressing cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer		Present		2B RERE , 2B merge
TS28: cerebellum molecular layer		Present		2B RERE , 2B merge
TS28: cerebellum Purkinje cell layer	Purkinje cell	Strong		2B RERE , 2B merge	Coexpressed with Calb1 in all Purkinje cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum		Present	Widespread	2C RERE , 2C merge	Positive cells were seen in the entire cerebellar region.
TS28: cerebellum Purkinje cell layer		Strong		2C merge , 2C RERE
TS28: cerebellum external granule cell layer		Present	Punctate	2C merge , 2C RERE	Less intense than seen in other regions of the cerebellum, and did not always counterstain with DAPI.
TS28: cerebellum external granule cell layer	cerebellar granule cell precursor	Absent		2C RERE , 2C merge	Not co-expressed with Pax6 in granule cell precursors.

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Present	Widespread	S1A E17.5	Expression was still maintained in the entire cerebellum.
TS25: cerebellum external granule cell layer	Present	Punctate	S1A E17.5	Less intense than seen in other regions of the cerebellum, and did not always counterstain with DAPI.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Widespread	S1A P7	Positive cells were seen in the entire cerebellar region.
TS28: cerebellum Purkinje cell layer	Strong		S1A P7
TS28: cerebellum external granule cell layer	Present	Punctate	S1A P7	Less intense than seen in other regions of the cerebellum, and did not always counterstain with DAPI.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: cerebellum external granule cell layer	cerebellar granule cell precursor	Absent		S1B right , S1B left	Not co-expressed with Pax6 in granule cell precursors.