About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:272622 Mochizuki K, et al., SETDB1 is essential for mouse primordial germ cell fate determination by ensuring BMP signaling. Development. 2018 Dec 3;145(23):dev164160
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8329538
Gene symbol: Smad9
Gene name: SMAD family member 9
Antibody: anti-Phospho-Smad1(Ser463/465)/ Smad5(Ser463/465)/ Smad9(Ser465/467) (D5B10) (#13820)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3A WT: embryonic day 7.5 (more )
Note: Triple labeled: green - Smad9 and Smad1 and Smad5; red - Prdm1 (assay MGI:8329534); cyan - T (assay MGI:8329535).
Structure Level Pattern Image Note
TS10: epiblast Present Regionally restricted 3A p-SMAD1/5/8 WT , 3A Merge WT Expression was detected in the posterior-proximal epiblast.
TS10: embryo mesoderm Present 3A p-SMAD1/5/8 WT , 3A Merge WT
Specimen 3A Setdb1 cKO: embryonic day 7.5; Edil3Tg(Sox2-cre)1Amc/0, Setdb1tm1.1Yshk/Setdb1tm1.1Yshk (more )
Note: Conditional mutant. Triple labeled: green - Smad9 and Smad1 and Smad5; red - Prdm1 (assay MGI:8329534); cyan - T (assay MGI:8329535).
Structure Level Pattern Image Note
TS10: epiblast Weak Regionally restricted 3A p-SMAD1/5/8 Setdb1 cKO , 3A Merge Setdb1 cKO Expression in the posterior-proximal epiblast was reduced in the Setdb1 cKO.
TS10: embryo mesoderm Weak Regionally restricted 3A p-SMAD1/5/8 Setdb1 cKO , 3A Merge Setdb1 cKO Reduced expression was also observed in the adjacent mesodermal cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
09/15/2026
MGI 6.29 		The Jackson Laboratory