Antibody:	anti-DSP (DP-1)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Weak	Regionally restricted	1a Stage 1 DSP+DAPI , 1a Stage 1 DSP	Low level expression in nucleus and cytoplasm. Minimal junctional expression.

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Weak	Regionally restricted	1a Stage 2 DSP+DAPI , 1a Stage 2 DSP	Faint expression. Cell-cell borders remain indistinct.

Structure	Level	Pattern	Image	Note
TS5: trophectoderm	Present	Regionally restricted	1a Stage 3 DSP inset , 1a Stage 3 DSP+DAPI , 1a Stage 3 DSP	Nuclear expression largely absent. Distinct junctional localization at many cell-cell contacts and membranes.

Structure	Level	Pattern	Image	Note
TS5: trophectoderm	Present	Regionally restricted	1a Stage 4 DSP , 1a Stage 4 DSP inset , 1a Stage 4 DSP+DAPI	Robust junctional localization of expression evident at most cell membranes.

Structure	Level	Pattern	Image	Note
TS6: trophectoderm	Present	Regionally restricted	1a Stage 5 DSP , 1a Stage 5 DSP+DAPI	Expression predominantly localized to junctions. Very little cytoplasmic expression.

Structure	Level	Pattern	Image	Note
TS6: trophectoderm	Present	Regionally restricted	1f Canonical Inset , 1f Canonical	Author report that junctions in later stage blastocyst adopt the canonical arrangement.

Structure	Level	Pattern	Image	Note
TS6: trophectoderm	Present	Regionally restricted	1f Non-canonical , 1f Non-canonical inset	Author report that junctions in later stage blastocyst adopt the canonical arrangement.