Ednrbem1(icre/ERT2)Eem
Endonuclease-mediated Allele Detail
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| Symbol: |
Ednrbem1(icre/ERT2)Eem |
| Name: |
endothelin receptor type B; endonuclease-mediated mutation 1, Edward E Morrisey |
| MGI ID: |
MGI:8255186 |
| Synonyms: |
Ednrbcre/ERT2 |
| Gene: |
Ednrb Location: Chr14:104052055-104081764 bp, - strand Genetic Position: Chr14, 53.05 cM
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| Alliance: |
Ednrbem1(icre/ERT2)Eem page
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| Allele Type: |
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Endonuclease-mediated (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Ednrbem1(icre/ERT2)Eem expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Ednrb (MGI:102720) |
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Mutation details: The endogenous stop codon of Ednrb was replaced with T2A-iCre/ERT2, followed by a 3xSTOP sequence (tgactgactgat), endogenous polyA signal, 3' regulatory elements, and FRT-flanked Neomycin resistance cassette, directed by a guide RNA (gRNA) targeting the genomic sequence. Double-stranded donor DNA containing the T2A-iCre/ERT2 construct (an optimized variant of Cre recombinase [iCre; improved with mammalian codon usage] fused to Cre-ER(T2) [a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain]) and flanking homologous arms was co-injected with gRNA and Cas9 protein into (C57BL/6 x 129S4/SvJae)F1 derived v6.5 mouse embryonic stem (ES) cells. Flp recombinase removed the frt-flanked neo cassette.
(J:101977)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Ednrb
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Ednrb Mutation: |
107 strains or lines available
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| Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
| All: |
1 reference(s) |
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Analysis Tools
