Tnnt1tm1.1(cre/ERT2)Hze
Targeted Allele Detail
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| Symbol: |
Tnnt1tm1.1(cre/ERT2)Hze |
| Name: |
troponin T1, skeletal, slow; targeted mutation 1.1, Hongkui Zeng |
| MGI ID: |
MGI:8245056 |
| Gene: |
Tnnt1 Location: Chr7:4507568-4518974 bp, - strand Genetic Position: Chr7, 2.6 cM
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| Alliance: |
Tnnt1tm1.1(cre/ERT2)Hze page
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| Allele Type: |
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Targeted (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tnnt1tm1.1(cre/ERT2)Hze expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting vector contained, from 5' to 3', a FRT3 site, a partial Tnnt2 sequence within exon 14, an internal ribosome entry site 2 sequence (IRES2; allows translation initiation in the middle of an mRNA sequence), a cre/ERT2 gene, a bovine growth hormone polyA sequence, an AttB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette, a FRT5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. In addition, several bp mutations were introduced into the first 30 nucleotides of the 3' UTR of Tnnt1, within exon 14. These mutated bp were included in the targeting vector to protect it from Crispr/Cas9 cleavage. This construct was electroporated into (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells along with cas9 protein and sgRNA(s). Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove a PGK/gb2 promoter-Neomycin resistance gene.
(J:101977)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Tnnt1
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Tnnt1 Mutation: |
4 strains or lines available
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| Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
| All: |
1 reference(s) |
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Analysis Tools
