Crhr1tm1.1(cre/GFP)Rpa
Targeted Allele Detail
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| Symbol: |
Crhr1tm1.1(cre/GFP)Rpa |
| Name: |
corticotropin releasing hormone receptor 1; targeted mutation 1.1, Richard D Palmiter |
| MGI ID: |
MGI:8244854 |
| Synonyms: |
Crhr1-frtFLEX-Cre |
| Gene: |
Crhr1 Location: Chr11:104023681-104066349 bp, + strand Genetic Position: Chr11, 67.77 cM
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| Alliance: |
Crhr1tm1.1(cre/GFP)Rpa page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:349993
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
Not Specified
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| Allele Type: |
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Targeted (Inducible, Recombinase) |
| Mutation: |
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Insertion
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Crhr1tm1.1(cre/GFP)Rpa expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Crhr1 (MGI:88498) |
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Mutation details: A FLP-dependent Cre recombinase was constructed by inserting a Mt1 gene intron within the open reading frame of mnCre:GFP, which has a Myc tag (m), followed by a nuclear localization signal (NLS, n), followed by Cre recombinase fused to green fluorescent protein (Cre:GFP), to generate a gene with two exons. Then the second exon was inverted and flanked by a pair of dissimilar
frt sites to make a double inverted orientation (DIO) construct (denoted as FLEX). The action of FLP recombinase inverts the second exon thereby allowing splicing to generate functional Cre recombinase. The construct was inserted in-frame to the Crhr1 gene. A neomycin selection cassette was removed via flp-mediated recombination.
(J:349993)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Crhr1
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Crhr1 Mutation: |
26 strains or lines available
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| Original: |
J:349993 Heymann G, et al., Synergy of Distinct Dopamine Projection Populations in Behavioral Reinforcement. Neuron. 2020 Mar 4;105(5):909-920.e5 |
| All: |
1 reference(s) |
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Analysis Tools
