Trpm3em2(cre/ERT2)Cya
Endonuclease-mediated Allele Detail
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| Symbol: |
Trpm3em2(cre/ERT2)Cya |
| Name: |
transient receptor potential cation channel, subfamily M, member 3; endonuclease-mediated mutation 2, Cyagen Biosciences |
| MGI ID: |
MGI:8235731 |
| Synonyms: |
Trpm3em1(P2A-EGFP-T2A-CreERT2) |
| Gene: |
Trpm3 Location: Chr19:22116410-22972774 bp, + strand Genetic Position: Chr19, 16.05 cM
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| Alliance: |
Trpm3em2(cre/ERT2)Cya page
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| Allele Type: |
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Endonuclease-mediated (Inducible, Recombinase, Reporter) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Trpm3em2(cre/ERT2)Cya expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The allele was generated via genome editing by replacing the TAA stop codon of the mouse endogenous regulatory elements. The 2A peptide linkage overcomes reduced protein activity and downstream gene expression typically associated with multi-gene constructs. Without tamoxifen treatment, CreERT2 recombinase primarily resides in the cytoplasm; nuclear translocation and recombination activity of CreERT2 only occur upon tamoxifen stimulation. When crossed with allele containing loxP sites, tamoxifen induction triggers Cre recombinase-mediated sequence recombination between loxP sites in Trpm3-positive cells of offspring. Beyond Cre-loxP gene recombination, this model enables EGFP-based fluorescent tracing.
(J:326541)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Trpm3
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Trpm3 Mutation: |
103 strains or lines available
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Analysis Tools
