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Leng8em2(icre)Cya
Endonuclease-mediated Allele Detail
Summary
Symbol: Leng8em2(icre)Cya
Name: leukocyte receptor cluster (LRC) member 8; endonuclease-mediated mutation 2, Cyagen Biosciences
MGI ID: MGI:8235640
Synonyms: Leng8em1(3xFLAG tag-EGFP-iCre)
Gene: Leng8  Location: Chr7:4140038-4151176 bp, + strand  Genetic Position: Chr7, 2.39 cM
Alliance: Leng8em2(icre)Cya page
Mutation
origin
Strain of Origin:  C57BL/6JCya
Mutation
description
Allele Type:    Endonuclease-mediated (Epitope tag, Recombinase, Reporter)
Mutation:    Insertion
 
Leng8em2(icre)Cya expression driven by 1 gene
 
Mutation details: The TAG stop codon was replaced with the 3xFLAG tag-3xEAAAK-EGFP-IRES-iCre cassette. Cre recombinase and enhanced green fluorescent protein (EGFP) are expressed under the regulatory control of the endogenous gene elements. The 3xFLAG tag is a tag consisting of three tandem FLAG epitopes (DYKDDDDK). When inserted into a target gene, it allows for efficient detection, localization, or purification of the target protein using anti-FLAG antibodies. (J:326541)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Leng8 (mouse)
Summary of all recombinase alleles driven by Leng8.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Leng8 Mutation:  38 strains or lines available
References
Original:  J:326541 Cyagen Biosciences Inc., Cyagen Biosciences Website. 2022;
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
08/05/2025
MGI 6.24 		The Jackson Laboratory