Calb1em4(cre*)Cya
Endonuclease-mediated Allele Detail
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| Symbol: |
Calb1em4(cre*)Cya |
| Name: |
calbindin 1; endonuclease-mediated mutation 4, Cyagen Biosciences |
| MGI ID: |
MGI:8235517 |
| Synonyms: |
Calb1em2(T2A-DHFR-EGFP-Cre) |
| Gene: |
Calb1 Location: Chr4:15881264-15906709 bp, + strand Genetic Position: Chr4, 6.66 cM
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| Alliance: |
Calb1em4(cre*)Cya page
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| Allele Type: |
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Endonuclease-mediated (Inducible degradation, Recombinase) |
| Mutation: |
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Insertion
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Calb1em4(cre*)Cya expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Calb1 (MGI:88248) |
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Mutation details: The TAG stop codon was replaced with the T2A-dgCre (DHFR-EGFP-Cre) cassette. Cre recombinase and enhanced green fluorescent protein (EGFP) are expressed under the regulatory control of the endogenous gene elements, without impacting the endogenous expression of the Calb1 gene. The DHFR (dihydrofolate reductase) fragment, derived from the N-terminal 159 amino acid s of E. coli ecDHFR, functions as a destabilizing domain (DD). In the absence of specific ligands like TMP (Trimethoprim), this fragment leads to the rapid proteasomal degradation of Cre recombinase. This mechanism allows for the temporal-specific induction of Cre recombinase activity by regulating DHFR-Cre stability with small molecules such as TMP. In addition to studies of Cre recombinase-mediated gene recombination, this model can also be used for EGFP fluorescent protein tracing studies.
(J:326541)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Calb1
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Calb1 Mutation: |
24 strains or lines available
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Analysis Tools
