Vps13b RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:339602 Montillot C, et al., Characterization of Vps13b-mutant mice reveals neuroanatomical and behavioral phenotypes with females less affected. Neurobiol Dis. 2023 Sep;185:106259
Assay type:	RT-PCR
MGI Accession ID:	MGI:8192831
Gene symbol:	Vps13b
Gene name:	vacuolar protein sorting 13B

Probe:	Vps13b Ex1/2-pF, Vps13b Ex1/2-pR
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Hprt or Gnas.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
Cortex	P w 19	TS28: cerebral cortex	Present (b)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
Hippocampus	P w 19	TS28: hippocampus	Present (b)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
Cerebellum	P w 19	TS28: cerebellum	Present (b)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
Pons	P w 19	TS28: pons	Present (c)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
Hypothalamus	P w 19	TS28: hypothalamus	Present (d)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
Heart	P w 19	TS28: heart	Present (e)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
Liver	P w 19	TS28: liver	Present (f)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
Kidney	P w 19	TS28: metanephros	Present (f)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
WAT	P w 19	TS28: white fat	Present (e)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
Muscle	P w 19	TS28: muscle tissue	Present (e)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
Lung	P w 19	TS28: lung	Present (f)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female

Notes:
(a) Consistent expression throughout. Expression profiles were consistent between males and females.
(b) Highest expression in cerebellum, hippocampus, cortex, liver, kidney and lung. In central nervous system, canonical transcript seems to be main transcript expressed.
(c) In central nervous system, canonical transcript seems to be main transcript expressed. In the pons, an additional putative mouse transcript consistent with transcript in corresponding human locus.
(d) In central nervous system, canonical transcript seems to be main transcript expressed.
(e) Authors report differential transcript distribution in peripheral system.
(f) Highest expression in cerebellum, hippocampus, cortex, liver, kidney and lung. Authors report differential transcript distribution in peripheral system.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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