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Tg(CAG-tdTomato*,-EGFP*,-cre,-EYFP*,-mCherry*,-flpo)iFlpBen
Transgene Detail
Summary
Symbol: Tg(CAG-tdTomato*,-EGFP*,-cre,-EYFP*,-mCherry*,-flpo)iFlpBen
Name: transgene insertion iFlp, Rui Benedito
MGI ID: MGI:8192471
Synonyms: FlpMosaics, Tg-iFlpMTomato-H2B-GFP-Cre/MYFP-H2B-Cherry-FlpO
Transgene: Tg(CAG-tdTomato*,-EGFP*,-cre,-EYFP*,-mCherry*,-flpo)iFlpBen  Location: unknown  Genetic Position: Chr3, Syntenic
Alliance: Tg(CAG-tdTomato*,-EGFP*,-cre,-EYFP*,-mCherry*,-flpo)iFlpBen page
Transgene
origin
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Transgene
description
Transgene Type:    Transgenic (Conditional ready, Recombinase, Reporter)
Mutation:    Insertion
 
Tg(CAG-tdTomato*,-EGFP*,-cre,-EYFP*,-mCherry*,-flpo)iFlpBen expression driven by 1 gene
 
Mutation detailsThe transgene contains a CAG promoter, FRT F3 site, FRT ST2 site, neomycin selection cassette, two polyadenylation sequences, FRT F3 site, Mb-Tomato gene (non-oligomerizing tandem-dimer DsRed fluorescent protein variant tdTomato with N-terminal 41 amino acid membrane-targeting tag sequence), 2A sequences, nuclear localized EGFP (H2B-GFP), V5 sequence, 2A site, cre recombinase gene, polyadenylation sequence, FRT ST2 site, MbYFP gene (monomeric enhanced yellow fluorescent protein [mYFPA206K] with N-terminal membrane-targeting tag sequence), 2A sequences, H2B-Cherry gene (monomeric cherry with 127 amino acid human histone 2B chromatin/nucleus tag and KOZAK sequence plus N-terminal HA tag sequence), 2A sequence, flpo recombinase gene, and polyadenylation sequence. (J:366224)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: CAG (chicken)
Summary of all recombinase alleles driven by CAG.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
References
Original:  J:366224 Garcia-Gonzalez I, et al., iFlpMosaics enable the multispectral barcoding and high-throughput comparative analysis of mutant and wild-type cells. Nat Methods. 2025 Feb;22(2):323-334
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory