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Cst7em3(folA/cre)Aduci
Endonuclease-mediated Allele Detail
Summary
Symbol: Cst7em3(folA/cre)Aduci
Name: cystatin F (leukocystatin); endonuclease-mediated mutation 3, Frank LaFerla
MGI ID: MGI:8163419
Synonyms: Cst7DD-Cre
Gene: Cst7  Location: Chr2:150412335-150420864 bp, + strand  Genetic Position: Chr2, 74.67 cM, cytoband G1-G3
Alliance: Cst7em3(folA/cre)Aduci page
Mutation
origin
Strain of Origin:  C57BL/6J
Mutation
description
Allele Type:    Endonuclease-mediated (Inducible, Recombinase)
Inducer:    trimethoprim
Mutation:    Insertion
 
Cst7em3(folA/cre)Aduci expression driven by 1 gene
 
Mutation details: sgRNAs (TMF1131-CCTGCATTTCCCCAGCCATG and TMF-1132-AAGCAGAATGGCCAGCCACA) were used to target sequences immediately downstream of the translation initiation codon in exon 1 of the cystatin F (leukocystatin) (Cst7) gene for insertion of a 1596 bp DNA sequence encoding a mutant destabilized domain from bacterial dihydrofolate reductase (ecDHFR) fused to a cre recombinase gene followed by a 2A oligopeptide (P2A) self-cleaving peptide, to mediate ribosomal skipping. (J:101977, J:363515)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Cst7 (mouse)
Summary of all recombinase alleles driven by Cst7.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cst7 Mutation:  20 strains or lines available
References
Original:  J:363515 Henningfield CM, et al., Generation of an Inducible Destabilized-Domain Cre Mouse Line to Target Disease Associated Microglia. Glia. 2025 Feb 23;
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory