Trpv1tm1.1(flp)Ttc
Targeted Allele Detail
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| Symbol: |
Trpv1tm1.1(flp)Ttc |
| Name: |
transient receptor potential cation channel, subfamily V, member 1; targeted mutation 1.1, Thomas Taylor-Clark |
| MGI ID: |
MGI:8161908 |
| Gene: |
Trpv1 Location: Chr11:73125118-73152068 bp, + strand Genetic Position: Chr11, 45.25 cM, cytoband B3
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| Alliance: |
Trpv1tm1.1(flp)Ttc page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:363352
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
C57BL/6J
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| Allele Type: |
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Targeted (Recombinase) |
| Mutation: |
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Insertion
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Trpv1tm1.1(flp)Ttc expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: 6. To develop a knock-in mouse that expresses the codon-optimized FlpO recombinase dependent on TRPV1 expression, the TGA stop codon was replaced with a 2A-Flp cassette. The targeting vector homology arms were generated by high fidelity Taq PCR using BAC clone RP23-30N20 and RP23-152H19 from the C57BL/6J library as template. The targeting vector was assembled with recombination sites and selection markers: Neomycin resistance gene (NeoR) flanked by self-deletion anchor (SDA) sites for positive selection and diphtheria toxin A fragment gene (DTA) for negative selection. The final version of the allele lacks the selection cassette.
(J:363352)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Trpv1
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Trpv1 Mutation: |
60 strains or lines available
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| Original: |
J:363352 Taylor-Clark T, Direct data submission for a flp recombinase knockin allele of Trpv1. MGI Direct Data Submission. 2025; |
| All: |
1 reference(s) |
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Analysis Tools
