Hprt1tm1.1(cre/ERT2)Alis
Targeted Allele Detail
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| Symbol: |
Hprt1tm1.1(cre/ERT2)Alis |
| Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 1.1, Adrian Liston |
| MGI ID: |
MGI:7867633 |
| Synonyms: |
Hprt1ERT2CreEGFP |
| Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
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| Alliance: |
Hprt1tm1.1(cre/ERT2)Alis page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:101977
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
C57BL/6
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| Allele Type: |
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Targeted (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Hprt1tm1.1(cre/ERT2)Alis expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Hprt1 (MGI:96217) |
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Mutation details: The targeting vector was sequentially assembled using BAC clone RP24-335G16 and RP23-412J16 from the C57BL/6 library with recombination sites and the Neo cassette selection cassette flanked by SDA (self-deletion anchor) sites. The TAA stop codon in exon 9 of the hypoxanthine phosphoribosyltransferase 1 (Hprt1) gene has been replaced with a T2A-CreERT2- P2A-EGFP cassette. A T2A self-cleaving peptide sequence was placed directly downstream of the Hprt1 coding sequence to separate Hprt1 and the CreER fusion protein. The subsequent P2A self-cleaving peptide separates the Hprt1 and CreER coding sequences. The neo cassette was removed by SDA.
(J:101977)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Hprt1
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Hprt1 Mutation: |
1280 strains or lines available
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| Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
| All: |
1 reference(s) |
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Analysis Tools
