Antibody:	anti-A2BP1-B (1734-2)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		4A	Expressed in Purkinje cell body and dendrites.
TS28: cerebellum granule cell layer	Strong		4A

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Strong		4B	myocardium

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Strong		4C
TS28: dentate gyrus	Absent		4C
TS28: hippocampus CA1	Absent		4C
TS28: hippocampus CA2	Absent		4C

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Strong		4D	Intense cytoplasmic staining extending into neurites was shown.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Strong		4E	Immunoreactivity was juxtanuclear, while the nuclei are not stained.

Structure	Level	Pattern	Image	Note
TS14: embryo mesenchyme	Strong		5A right
TS14: heart	Strong		5A right
TS14: hematopoietic system	Strong		5A right
TS14: central nervous system	Absent		5A right

Structure	Level	Pattern	Image	Note
TS20: heart	Strong		5B
TS20: intestine epithelium	Absent		5B
TS20: liver	Absent		5B

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Present	Punctate	5C	A punctate pattern was observed at this stage in some cells of the developing vertebral column, while other surrounding cells remained unlabeled.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	5D	Expressed in large alpha-motoneuron progenitors in the anterior region.

Structure	Level	Pattern	Image	Note
TS23: intestine epithelium	Present		5E	Rapidly increased expression in epithelial tissues. Labeling was also prominent in the intestinal mucosa.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Strong		5F right
TS24: heart	Strong		5F right
TS24: spinal cord	Strong		5F right

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex ventricular layer	Trace		5G	Little expression.
TS24: cerebral cortex intermediate zone	Present	Regionally restricted	5G	Expressed only in a subset of cells.
TS24: cortical plate	Present	Regionally restricted	5G	Strongest staining was seen in the neopallial cortex, which contains already differentiated neurons.

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Strong		5H	Expressed in the motoneurons.
TS24: skeletal muscle	Strong		5H
TS24: vertebra	Strong		5H	Expressed in the chondro-/osteoblasts in the developing vertebral column.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Strong		5I	Intense staining of large neurons was seen.

Structure	Level	Pattern	Image	Note
TS28: brainstem	Present			Neuronal cell types rather than glial cells where preferentially labeled.
TS28: cerebellum	Present			Neuronal cell types rather than glial cells where preferentially labeled.
TS28: cerebral cortex	Present			Neuronal cell types rather than glial cells where preferentially labeled.
TS28: hippocampus	Present			Neuronal cell types rather than glial cells where preferentially labeled.
TS28: metanephros	Present
TS28: heart	Strong

Structure	Level	Pattern	Image	Note
TS28: placenta	Strong

Structure	Level	Pattern	Image	Note
TS16: heart	Strong
TS16: hematopoietic system	Strong

Structure	Level	Pattern	Image	Note
TS18: heart	Strong
TS18: hematopoietic system	Strong

Structure	Level	Pattern	Image	Note
TS20: hematopoietic system	Strong
TS20: spinal cord	Present	Regionally restricted		Expressed in the anterior portion.
TS20: corpus striatum	Strong	Punctate		A punctate staining pattern, some cells were strongly immunoreactive while others remained unlabeled.
TS20: esophagus epithelium	Absent
TS20: gut epithelium	Absent
TS20: liver and biliary system epithelium	Absent
TS20: renal vesicle	Absent

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present	Punctate		Staining was highly selective.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Strong
TS23: cerebral cortex ventricular layer	Strong
TS23: hematopoietic system	Strong
TS23: spinal cord	Strong
TS23: lung	Present			Strikingly increased expression.
TS23: skin	Present			Strikingly increased expression.
TS23: cerebral cortex	Strong	Widespread		Expression was found in all 3 layers.
TS23: cerebellum	Present	Punctate		Staining was highly selective.

Structure	Level	Pattern	Image	Note
TS24: hematopoietic system	Strong
TS24: cerebellum	Present	Punctate		Staining was highly selective.