Evc2 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:316640 Badri MK, et al., Expression of Evc2 in craniofacial tissues and craniofacial bone defects in Evc2 knockout mouse. Arch Oral Biol. 2016 Aug;68:142-52
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7861443
Gene symbol:	Evc2
Gene name:	EvC ciliary complex subunit 2

Reporter:	lacZ
Antibody:	anti-beta-galactosidase (US Biological)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

Specimen 2A: embryonic day 15.5; Evc2^tm1.1Mis/Evc2^tm1.1Mis (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS23: Meckel's cartilage	chondrocyte	Present		2A	Expression is in chondrocytes.
TS23: maxillary sinus mesenchyme	chondrocyte	Present		2A	Expression is in chondrocytes.
TS23: nasal capsule	chondrocyte	Present		2A	Expression is in chondrocytes.
TS23: basioccipital bone	chondrocyte	Present	Regionally restricted	2A	Expression is in chondrocytes in cranial suture 2.
TS23: sphenoid bone	chondrocyte	Present	Regionally restricted	2A	Expression is in chondrocytes in cranial suture 2.
TS23: secondary palate		Present	Regionally restricted	2A	Expression is in mesenchymal cells of the posterior palate.
TS23: chondrocranium		Present	Regionally restricted	2A	Expression is in mesenchymal cells in cranial suture 1.

Specimen 2C: embryonic day 15.5; Evc2^tm1.1Mis/Evc2^tm1.1Mis (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS23: nasal capsule	chondrocyte	Present		2C	Expression is in chondrocytes.

Specimen 2E: embryonic day 15.5; Evc2^tm1.1Mis/Evc2^tm1.1Mis (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS23: maxillary sinus mesenchyme	chondrocyte	Present		2E	Expression is in chondrocytes.

Specimen 2G: embryonic day 15.5; Evc2^tm1.1Mis/Evc2^tm1.1Mis (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS23: Meckel's cartilage	chondrocyte	Present		2G	Expression is in chondrocytes.

Specimen 2I: embryonic day 15.5; Evc2^tm1.1Mis/Evc2^tm1.1Mis (more

)

Structure	Level	Pattern	Image	Note
TS23: secondary palate	Present	Regionally restricted	2I	Expression is in mesenchymal cells of the posterior palate.

Specimen 2K: embryonic day 15.5; Evc2^tm1.1Mis/Evc2^tm1.1Mis (more

)

Structure	Level	Pattern	Image	Note
TS23: chondrocranium	Present	Regionally restricted	2K	Expression is in mesenchymal cells in cranial suture 1.

Specimen 2M: embryonic day 15.5; Evc2^tm1.1Mis/Evc2^tm1.1Mis (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS23: chondrocranium	chondrocyte	Present	Regionally restricted	2M	Expression is in chondrocytes in cranial suture 2.

Specimen 2O: embryonic day 18.5; Evc2^tm1.1Mis/Evc2^tm1.1Mis (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: basioccipital bone	hypertrophic chondrocyte	Present	Regionally restricted	2O	Expression is in hypertrophic chondrocytes in the spheno-occipital synchondrosis.
TS26: sphenoid bone	hypertrophic chondrocyte	Present	Regionally restricted	2O	Expression is in hypertrophic chondrocytes in the spheno-occipital synchondrosis.

Specimen 6B: postnatal week 3; Evc2^tm1.1Mis/Evc2⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: nasal septum	hypertrophic chondrocyte	Present		6B	Expression is in hypertrophic chondrocytes.

Specimen 6D: postnatal week 3; Evc2^tm1.1Mis/Evc2^tm1.1Mis (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: nasal septum	hypertrophic chondrocyte	Present		6D	Expression is in hypertrophic chondrocytes.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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