Tg(Rr27-HBB)del-2Keij
Transgene Detail
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| Symbol: |
Tg(Rr27-HBB)del-2Keij |
| Name: |
transgene insertion del-2, Keiji Tanimoto |
| MGI ID: |
MGI:7860834 |
| Synonyms: |
del-2 |
| Transgene: |
Tg(Rr27-HBB)del-2Keij Location: unknown
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| Alliance: |
Tg(Rr27-HBB)del-2Keij page
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| Transgene Type: |
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Transgenic (Conditional ready, Humanized sequence, Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Rr27-HBB)del-2Keij expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
HBB (3043) |
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Mutation details: The transgene insert was generated as follows: two differently modified mouse H19 ICR sequences (del-2 and del-3, each flanked by loxP5171 and loxP2272 sites) were inserted in reverse orientation into human HBB cluster-containing YAC A201F4.3 immediately downstream of the LCR and upstream of the HBB cluster. Subsequent Cre-mediated recombination created separate single del-2 and del-3 transgene alleles. The modifications involve the deletion of the 5' end of the ICR and all but the last 60 bp (del-2) or 120 (del-3) of a 478 bp sequence close to the 5' end that confers post-fertilization paternal methylation to the ICR.
(J:303109)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:303109 Matsuzaki H, et al., Recapitulation of gametic DNA methylation and its post-fertilization maintenance with reassembled DNA elements at the mouse Igf2/H19 locus. Epigenetics Chromatin. 2020 Jan 14;13(1):2 |
| All: |
1 reference(s) |
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Analysis Tools
